A gene cluster that regulates both heterocyst differentiation and pattern formation in Anabaena sp strain PCC 7120
文献类型：期刊论文
| 作者 | Zhang, Wei; Du, Ye; Khudyakov, Ivan; Fan, Qing; Gao, Hong; Ning, Degang; Wolk, C. Peter; Xu, Xudong |
| 刊名 | MOLECULAR MICROBIOLOGY
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 66期号:6页码:1429-1443 |
| 关键词 | CYANOBACTERIUM ANABAENA |
| ISSN号 | 0950-382X |
| 通讯作者 | Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | Wild-type Anabaena sp. strain PCC 7120, a filamentous nitrogen-fixing cyanobacterium, produces single heterocysts at semi-regular intervals. asr0100 (patU5) and alr0101 (patU3) are homologous to the 5' and 3' portions of patU of Nostoc punctiforme. alr0099 (hetZ) overlaps the 5' end of patU5. hetZ, patU5 and patU3 were all upregulated, or expressed specifically, in proheterocysts and heterocysts. Mutants of hetZ showed delayed or no heterocyst differentiation. In contrast, a patU3 mutation produced a multiple contiguous heterocyst (Mch) phenotype and restored the formation of otherwise lost intercalary heterocysts in a patA background. Decreasing the expression of patU3 greatly increased the frequency of heterocysts in a mini-patS strain. Two promoter regions and two principal, corresponding transcripts were detected in the hetZ-patU5-patU3 region. Transcription of hetZ was upregulated in a hetZ mutant and downregulated in a patU3 mutant. When mutants hetZ::C.K2 and hetZ::Tn5-1087b were nitrogen-deprived, P-hetC-gfp was very weakly expressed, and in hetZ::Tn5-1087b, P-hetR-gfp was relatively strongly expressed in cells that had neither a regular pattern nor altered morphology. We conclude that the hetZ-patU5-patU3 cluster plays an important role in co-ordination of heterocyst differentiation and pattern formation. The presence of homologous clusters in filamentous genera without heterocysts is suggestive of a more general role. |
| 英文摘要 | Wild-type Anabaena sp. strain PCC 7120, a filamentous nitrogen-fixing cyanobacterium, produces single heterocysts at semi-regular intervals. asr0100 (patU5) and alr0101 (patU3) are homologous to the 5' and 3' portions of patU of Nostoc punctiforme. alr0099 (hetZ) overlaps the 5' end of patU5. hetZ, patU5 and patU3 were all upregulated, or expressed specifically, in proheterocysts and heterocysts. Mutants of hetZ showed delayed or no heterocyst differentiation. In contrast, a patU3 mutation produced a multiple contiguous heterocyst (Mch) phenotype and restored the formation of otherwise lost intercalary heterocysts in a patA background. Decreasing the expression of patU3 greatly increased the frequency of heterocysts in a mini-patS strain. Two promoter regions and two principal, corresponding transcripts were detected in the hetZ-patU5-patU3 region. Transcription of hetZ was upregulated in a hetZ mutant and downregulated in a patU3 mutant. When mutants hetZ::C.K2 and hetZ::Tn5-1087b were nitrogen-deprived, P-hetC-gfp was very weakly expressed, and in hetZ::Tn5-1087b, P-hetR-gfp was relatively strongly expressed in cells that had neither a regular pattern nor altered morphology. We conclude that the hetZ-patU5-patU3 cluster plays an important role in co-ordination of heterocyst differentiation and pattern formation. The presence of homologous clusters in filamentous genera without heterocysts is suggestive of a more general role. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Microbiology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Microbiology |
| 关键词[WOS] | CYANOBACTERIUM ANABAENA ; NOSTOC-PUNCTIFORME ; RESPONSE REGULATOR ; HETR FUNCTION ; EXPRESSION ; PATS ; NTCA ; DNA ; MAINTENANCE ; NITROGENASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251252200012 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8354]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.St Petersburg State Univ, All Russia Res Inst Agr Microbiol, St Petersburg, Russia 3.St Petersburg State Univ, Biol Res Inst, St Petersburg, Russia 4.Michigan State Univ, MSU DOE Plant Res Lab, E Lansing, MI 48824 USA 5.Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Wei,Du, Ye,Khudyakov, Ivan,et al. A gene cluster that regulates both heterocyst differentiation and pattern formation in Anabaena sp strain PCC 7120[J]. MOLECULAR MICROBIOLOGY,2007,66(6):1429-1443. |
| APA | Zhang, Wei.,Du, Ye.,Khudyakov, Ivan.,Fan, Qing.,Gao, Hong.,...&Xu, Xudong.(2007).A gene cluster that regulates both heterocyst differentiation and pattern formation in Anabaena sp strain PCC 7120.MOLECULAR MICROBIOLOGY,66(6),1429-1443. |
| MLA | Zhang, Wei,et al."A gene cluster that regulates both heterocyst differentiation and pattern formation in Anabaena sp strain PCC 7120".MOLECULAR MICROBIOLOGY 66.6(2007):1429-1443. |
入库方式： OAI收割
来源：水生生物研究所
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