Identification of differentially expressed genes from the cross-subfamily cloned embryos derived from zebrafish nuclei and rare minnow enucleated eggs
文献类型：期刊论文
| 作者 | Pei, D. S.; Sun, Y. H.; Chen, S. P.; Wang, Y. P.; Hu, W.; Zhu, Z. Y. |
| 刊名 | THERIOGENOLOGY
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 68期号:9页码:1282-1291 |
| 关键词 | nuclear transfer nuclear reprogramming nucleo-cytoplasmic interaction suppression subtractive hybridization cross-subfamily cloned embryos |
| ISSN号 | 0093-691X |
| 通讯作者 | Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Cross-species nuclear transfer (NT) has been used to retain the genetic viability of a species near extinction. However, unlike intra-species NT, most embryos produced by cross-species NT were unable to develop to later stages due to incompatible nucleocytoplasmic interactions between the donor nuclei and the recipient cytoplasm from different species. To study the early nucleocytoplasmic interaction in cross-species NT, two laboratory fish species (zebrafish and rare minnow) from different subfamilies were used to generate cross-subfamily NT embryos in the present study. Suppression subtractive hybridization (SSH) was performed to screen out differentially expressed genes from the forward and reverse subtractive cDNA libraries. After dot blot and real-time PCR analysis, 80 of 500 randomly selective sequences were proven to be differentially expressed in the cloned embryos. Among them, 45 sequences shared high homology with 28 zebrafish known genes, and 35 sequences were corresponding to 22 novel expressed sequence tags (ESTs). Based on functional clustering and literature mining analysis, up-and down-regulated genes in the cross-subfamily cloned embryos were mostly relevant to transcription and translation initiation, cell cycle regulation, protein binding, etc. To our knowledge, this is the first report on the determination of genes involved in the early development of cross-species NT embryos of fish. (C) 2007 Elsevier Inc. All rights reserved. |
| 英文摘要 | Cross-species nuclear transfer (NT) has been used to retain the genetic viability of a species near extinction. However, unlike intra-species NT, most embryos produced by cross-species NT were unable to develop to later stages due to incompatible nucleocytoplasmic interactions between the donor nuclei and the recipient cytoplasm from different species. To study the early nucleocytoplasmic interaction in cross-species NT, two laboratory fish species (zebrafish and rare minnow) from different subfamilies were used to generate cross-subfamily NT embryos in the present study. Suppression subtractive hybridization (SSH) was performed to screen out differentially expressed genes from the forward and reverse subtractive cDNA libraries. After dot blot and real-time PCR analysis, 80 of 500 randomly selective sequences were proven to be differentially expressed in the cloned embryos. Among them, 45 sequences shared high homology with 28 zebrafish known genes, and 35 sequences were corresponding to 22 novel expressed sequence tags (ESTs). Based on functional clustering and literature mining analysis, up-and down-regulated genes in the cross-subfamily cloned embryos were mostly relevant to transcription and translation initiation, cell cycle regulation, protein binding, etc. To our knowledge, this is the first report on the determination of genes involved in the early development of cross-species NT embryos of fish. (C) 2007 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Reproductive Biology; Veterinary Sciences |
| 类目[WOS] | Reproductive Biology ; Veterinary Sciences |
| 研究领域[WOS] | Reproductive Biology ; Veterinary Sciences |
| 关键词[WOS] | SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; GOBIOCYPRIS-RARUS ; TRANSLATION ; RESISTANCE ; PATTERNS ; CALVES ; CELLS ; PCR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251030700010 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8360]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pei, D. S.,Sun, Y. H.,Chen, S. P.,et al. Identification of differentially expressed genes from the cross-subfamily cloned embryos derived from zebrafish nuclei and rare minnow enucleated eggs[J]. THERIOGENOLOGY,2007,68(9):1282-1291. |
| APA | Pei, D. S.,Sun, Y. H.,Chen, S. P.,Wang, Y. P.,Hu, W.,&Zhu, Z. Y..(2007).Identification of differentially expressed genes from the cross-subfamily cloned embryos derived from zebrafish nuclei and rare minnow enucleated eggs.THERIOGENOLOGY,68(9),1282-1291. |
| MLA | Pei, D. S.,et al."Identification of differentially expressed genes from the cross-subfamily cloned embryos derived from zebrafish nuclei and rare minnow enucleated eggs".THERIOGENOLOGY 68.9(2007):1282-1291. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。