Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC
文献类型：期刊论文
| 作者 | Fang, Huaifang; Yang, Fangxing; Sun, Junling; Zeng, Zhaorui; Xu, Ying |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2007-10-01 |
| 卷号 | 28期号:20页码:3697-3704 |
| 关键词 | MEKC neutral analyte pressure-assisted field-amplified sample injection |
| ISSN号 | 0173-0835 |
| 通讯作者 | Zeng, ZR, Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China |
| 中文摘要 | This paper describes a novel method that applies pressure-assisted field-amplified sample injection with reverse migrating micelles (PA-FASI-RMM) for the online concentration of neutral analytes in MEKC with a low-pH BGE. After injection of a plug of water into the separation capillary, negative voltage and positive pressure were simultaneously applied to initialize PA-FASI-RMM injection. The hydrodynamic flow generated by the positive pressure compensated the reverse EOF in the water plug and allowed the water plug to remain in the capillary during FASI with reverse migrating micelles (FASI-RMM) to obtain a much longer injection time than usual, which improved stacking efficiency greatly. Equations describing this injection mode were introduced and were supported by experimental results. For a 450-s online PA-FASI-RMM injection, three orders of magnitude sample enhancement in terms of peak area could be observed for the steroids and an achievement of detection limits was between 1 and 10 ng/mL. |
| 英文摘要 | This paper describes a novel method that applies pressure-assisted field-amplified sample injection with reverse migrating micelles (PA-FASI-RMM) for the online concentration of neutral analytes in MEKC with a low-pH BGE. After injection of a plug of water into the separation capillary, negative voltage and positive pressure were simultaneously applied to initialize PA-FASI-RMM injection. The hydrodynamic flow generated by the positive pressure compensated the reverse EOF in the water plug and allowed the water plug to remain in the capillary during FASI with reverse migrating micelles (FASI-RMM) to obtain a much longer injection time than usual, which improved stacking efficiency greatly. Equations describing this injection mode were introduced and were supported by experimental results. For a 450-s online PA-FASI-RMM injection, three orders of magnitude sample enhancement in terms of peak area could be observed for the steroids and an achievement of detection limits was between 1 and 10 ng/mL. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 学科主题 | Biochemical Research Methods; Chemistry ; Analytical |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | CAPILLARY-ZONE-ELECTROPHORESIS ; SELECTIVE EXHAUSTIVE INJECTION ; STACKING NEUTRAL ANALYTES ; ELECTROKINETIC CHROMATOGRAPHY ; ONLINE CONCENTRATION ; HIGH-SALT ; OPTIMIZATION ; SPECTROMETRY ; ENHANCEMENT ; SEPARATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000250657100014 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8386]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol, Wuhan, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Huaifang,Yang, Fangxing,Sun, Junling,et al. Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC[J]. ELECTROPHORESIS,2007,28(20):3697-3704. |
| APA | Fang, Huaifang,Yang, Fangxing,Sun, Junling,Zeng, Zhaorui,&Xu, Ying.(2007).Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC.ELECTROPHORESIS,28(20),3697-3704. |
| MLA | Fang, Huaifang,et al."Pressure-assisted field-amplified sample injection with reverse migrating micelles for analyzing trace steroids in MEKC".ELECTROPHORESIS 28.20(2007):3697-3704. |
入库方式： OAI收割
来源：水生生物研究所
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