Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio
文献类型：期刊论文
| 作者 | Wang, Y.; Hu, W.; Liu, W.-Y.; Wang, Y.-P.; Zhu, Z.-Y. |
| 刊名 | JOURNAL OF FISH BIOLOGY
 |
| 出版日期 | 2007-10-01 |
| 卷号 | 71期号:4页码:1082-1094 |
| ISSN号 | 0022-1112 |
| 关键词 | alternative splicing common carp gonadotropin signal peptide |
| 通讯作者 | Hu, W, Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China |
| 中文摘要 | In this study, an alternative splicing transcript GtH-alpha 291 was identified by RT-PCR, which is 291 nt and exists not only in the pituitary but also in the ovary in common carp Cyprinus carpio. The analysis of GtH-alpha 291 amino acid sequence by the SignalP server predicted that the 'missing segment' might characterize as a signal peptide. In the secretion experiment, GtH-alpha 357 subunit could be secreted out of HeLa cells while GtH-alpha 291 could not, which confirmed the prediction. Co-immunoprecipitation assay proved that GtH-alpha 291 subunit is able to interact with both FSH-beta and LH-beta as GtH-alpha 357 does. This is the first report concerning an alternative splicing transcript of a GtH alpha subunit. Further studies are necessary to elucidate the specific role of this variant in the regulation of gonadal development and sexual maturation. (c) 2007 The Authors. |
| 英文摘要 | In this study, an alternative splicing transcript GtH-alpha 291 was identified by RT-PCR, which is 291 nt and exists not only in the pituitary but also in the ovary in common carp Cyprinus carpio. The analysis of GtH-alpha 291 amino acid sequence by the SignalP server predicted that the 'missing segment' might characterize as a signal peptide. In the secretion experiment, GtH-alpha 357 subunit could be secreted out of HeLa cells while GtH-alpha 291 could not, which confirmed the prediction. Co-immunoprecipitation assay proved that GtH-alpha 291 subunit is able to interact with both FSH-beta and LH-beta as GtH-alpha 357 does. This is the first report concerning an alternative splicing transcript of a GtH alpha subunit. Further studies are necessary to elucidate the specific role of this variant in the regulation of gonadal development and sexual maturation. (c) 2007 The Authors. |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | PITUITARY GLYCOPROTEIN HORMONES ; FOLLICLE-STIMULATING-HORMONE ; PRE-MESSENGER-RNA ; ENCODING 2 TYPES ; SALMON GONADOTROPINS ; NUCLEOTIDE-SEQUENCE ; MOLECULAR-CLONING ; SIGNAL PEPTIDES ; RECEPTOR GENE ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000250265800010 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8392]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Y.,Hu, W.,Liu, W.-Y.,et al. Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio[J]. JOURNAL OF FISH BIOLOGY,2007,71(4):1082-1094. |
| APA | Wang, Y.,Hu, W.,Liu, W.-Y.,Wang, Y.-P.,&Zhu, Z.-Y..(2007).Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio.JOURNAL OF FISH BIOLOGY,71(4),1082-1094. |
| MLA | Wang, Y.,et al."Identification and characterization of a novel splice variant of gonadotropin alpha subunit in the common carp Cyprinus carpio".JOURNAL OF FISH BIOLOGY 71.4(2007):1082-1094. |
入库方式： OAI收割
来源：水生生物研究所
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