Cloning, expression and characterization of aerolysin from Aeromonas hydrophila in Escherichia coli
文献类型：期刊论文
| 作者 | Zhu, Daling ; Li, Aihua ; Wang, Jianguo ; Li, Ming ; Cai, Taozhen |
| 刊名 | INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
 |
| 出版日期 | 2007 |
| 卷号 | 44期号:4页码:204-208 |
| 关键词 | Aeromonas hydrophila cloning aerA gene prokaryotic expression recombinant protein AerA-W hemolytic activity |
| 通讯作者 | Zhu, DL, Wuhan Univ, Chinese Acad Sci, Inst Hydrobiol, Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Aerolysin is a toxin (protein in nature) secreted by the strains of Aeromonas spp. and plays all important role in the virulence of Aeromonas strains. It has also found several applications such as for detection of glycosylphosphatidylinositol (GPI)-anchored proteins etc. A. hydrophila is a ubiquitous Gram-negative bacterium which causes frequent harm to the aquaculture. To obtain a significant amount of recombinant aerolysin in the active form, in this study, we expressed the aerolysin in E. Coli Under the control of T7 RNase promoter. The coding region (AerA-W) of the aerA gene of A. hydrophila XS91-4-1. excluding partial coding region of the signal peptide was cloned into the vector pET32a and then transformed into E. coli b121. After optimizing the expression conditions, the recombinant protein AerA-W was expressed in a soluble form and purified using His-Bind resin affinity chromatography. Recombinant aerolysin showed hemolytic activity in the agar diffusive hemolysis test. Western blot analysis demonstrated good antigenicity of the recombinant protein. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8398]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 推荐引用方式 GB/T 7714 | Zhu, Daling,Li, Aihua,Wang, Jianguo,et al. Cloning, expression and characterization of aerolysin from Aeromonas hydrophila in Escherichia coli[J]. INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,2007,44(4):204-208. |
| APA | Zhu, Daling,Li, Aihua,Wang, Jianguo,Li, Ming,&Cai, Taozhen.(2007).Cloning, expression and characterization of aerolysin from Aeromonas hydrophila in Escherichia coli.INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS,44(4),204-208. |
| MLA | Zhu, Daling,et al."Cloning, expression and characterization of aerolysin from Aeromonas hydrophila in Escherichia coli".INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS 44.4(2007):204-208. |
入库方式： OAI收割
来源：水生生物研究所
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