Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection
文献类型：期刊论文
| 作者 | Lu, Ai-Jun; Dong, Cai-Wen; Du, Chang-Sheng; Zhang, Qi-Ya |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 23期号:3页码:601-613 |
| 关键词 | voltage-dependent anion channel inductive expression Paralichthys olivaceus virus infection apoptosis |
| ISSN号 | 1050-4648 |
| 通讯作者 | Zhang, QY, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China |
| 中文摘要 | Voltage-dependent anion channel (VDAC, also known as mitochondrial porin) is acknowledged to play an important role in stress-induced mammalian apoptosis. In this study, Paralichthys olivaceus VDAC (PoVDAC) gene was identified as a virally induced gene from Scophthalmus Maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). The full length of PoVDAC cDNA is 1380 bp with an open reading frame of 852 bp encoding a 283 amino acid protein. The deduced PoVDAC contains one alpha-helix, 13 transmembrane beta-strands and one eukaryotic mitochondrial porin signature motif. Constitutive expression of PoVDAC was confirmed in all tested tissues by real-time PCR. Further expression analysis revealed PoVDAC mRNA was upregulated by viral infection. We prepared fish antiserum against recombinant VDAC proteins and detected the PoVDAC in heart lysates from flounder as a 32 kDa band on western blot. Overexpression of PoVDAC in fish cells induced apoptosis. Immunofluoresence localization indicated that the significant distribution changes of PoVDAC have occurred in virus-induced apoptotic cells. This is the first report on the inductive expression of VDAC by viral infection, suggesting that PoVDAC might be mediated flounder antiviral immune response through induction of apoptosis. (c) 2007 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Voltage-dependent anion channel (VDAC, also known as mitochondrial porin) is acknowledged to play an important role in stress-induced mammalian apoptosis. In this study, Paralichthys olivaceus VDAC (PoVDAC) gene was identified as a virally induced gene from Scophthalmus Maximus Rhabdovirus (SMRV)-infected flounder embryonic cells (FEC). The full length of PoVDAC cDNA is 1380 bp with an open reading frame of 852 bp encoding a 283 amino acid protein. The deduced PoVDAC contains one alpha-helix, 13 transmembrane beta-strands and one eukaryotic mitochondrial porin signature motif. Constitutive expression of PoVDAC was confirmed in all tested tissues by real-time PCR. Further expression analysis revealed PoVDAC mRNA was upregulated by viral infection. We prepared fish antiserum against recombinant VDAC proteins and detected the PoVDAC in heart lysates from flounder as a 32 kDa band on western blot. Overexpression of PoVDAC in fish cells induced apoptosis. Immunofluoresence localization indicated that the significant distribution changes of PoVDAC have occurred in virus-induced apoptotic cells. This is the first report on the inductive expression of VDAC by viral infection, suggesting that PoVDAC might be mediated flounder antiviral immune response through induction of apoptosis. (c) 2007 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | CELL-DEATH ; DROSOPHILA-MELANOGASTER ; CYTOCHROME-C ; APOPTOSIS ; IDENTIFICATION ; MITOCHONDRIA ; PROTEINS ; FAMILY ; LOCALIZATION ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000248509800011 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8438]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Chinese Acad Sci, Wuhan 430072, Peoples R China 2.Xuzhou Normal Univ, Sch Life Sci, Xuzhou 221116, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Ai-Jun,Dong, Cai-Wen,Du, Chang-Sheng,et al. Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2007,23(3):601-613. |
| APA | Lu, Ai-Jun,Dong, Cai-Wen,Du, Chang-Sheng,&Zhang, Qi-Ya.(2007).Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection.FISH & SHELLFISH IMMUNOLOGY,23(3),601-613. |
| MLA | Lu, Ai-Jun,et al."Characterization and expression analysis of Paralichthys olivaceus voltage-dependent anion channel (VDAC) gene in response to virus infection".FISH & SHELLFISH IMMUNOLOGY 23.3(2007):601-613. |
入库方式： OAI收割
来源：水生生物研究所
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