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Apoptosis induction on human hepatoma cells Hep G2 of decabrominated diphenyl ether (PBDE-209)
文献类型：期刊论文
| 作者 | Hu, Xiao-Zhong; Xu, Ying; Hu, De-Cong; Hui, Yang; Yang, Fang-Xing |
| 刊名 | TOXICOLOGY LETTERS
 |
| 出版日期 | 2007-06-15 |
| 卷号 | 171期号:1-2页码:19-28 |
| 关键词 | PBDE-209 hep G2 cells cell viability apoptosis LDH release reactive oxygen species |
| ISSN号 | 0378-4274 |
| 通讯作者 | Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Polybrominated diphenyl ethers (PBDEs) are an important class of halogenated organic brominated flame retardants. Because of their presence in abiotic and biotic environments widely and their structural similarity to polychlorinated biphenyls (PCBs), concern has been raised on their possible adverse health effects to humans. This study was designed to determine the anti-proliferative, apoptotic properties of decabrominated diphenyl ether (PBDE-209), using a human hepatoma Hep G2 line as a model system. Hep G2 cells were cultured in the presence of PBDE-209 at various concentrations (1.0-100.0 mu mol/L) for 72 h and the percentage of cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The results showed that PBDE-209 inhibited the cells viability in time and concentration-dependent characteristics at concentrations (10.0-100.0 mu mol/L). We found that anti-proliferative effect of PBDE-209 was associated with apoptosis on Hep G2 cells by determinations of morphological changes, cell cycle and apoptosis. Mechanism study showed that PBDE-209 could increase the generation of intracellular reactive oxygen species (ROS) concentration-dependently. Antioxidant N-acetylcyteine partially inhibited the increase of ROS. The mechanism for its hepatoma-inhibitory effects was the induction of cellular apoptosis through ROS generation. In addition, activity of lactate dehydrogenase (LDH) release increased when the cells incubated with PBDE-209 at various concentrations and times. These results suggested that PBDE-209 had the toxicity activity of anti-proliferation and induction of apoptosis in tumor cells in vitro. (c) 2007 Elsevier Ireland Ltd. All rights reserved. |
| 英文摘要 | Polybrominated diphenyl ethers (PBDEs) are an important class of halogenated organic brominated flame retardants. Because of their presence in abiotic and biotic environments widely and their structural similarity to polychlorinated biphenyls (PCBs), concern has been raised on their possible adverse health effects to humans. This study was designed to determine the anti-proliferative, apoptotic properties of decabrominated diphenyl ether (PBDE-209), using a human hepatoma Hep G2 line as a model system. Hep G2 cells were cultured in the presence of PBDE-209 at various concentrations (1.0-100.0 mu mol/L) for 72 h and the percentage of cell viability was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The results showed that PBDE-209 inhibited the cells viability in time and concentration-dependent characteristics at concentrations (10.0-100.0 mu mol/L). We found that anti-proliferative effect of PBDE-209 was associated with apoptosis on Hep G2 cells by determinations of morphological changes, cell cycle and apoptosis. Mechanism study showed that PBDE-209 could increase the generation of intracellular reactive oxygen species (ROS) concentration-dependently. Antioxidant N-acetylcyteine partially inhibited the increase of ROS. The mechanism for its hepatoma-inhibitory effects was the induction of cellular apoptosis through ROS generation. In addition, activity of lactate dehydrogenase (LDH) release increased when the cells incubated with PBDE-209 at various concentrations and times. These results suggested that PBDE-209 had the toxicity activity of anti-proliferation and induction of apoptosis in tumor cells in vitro. (c) 2007 Elsevier Ireland Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Toxicology |
| 类目[WOS] | Toxicology |
| 研究领域[WOS] | Toxicology |
| 关键词[WOS] | BROMINATED FLAME RETARDANTS ; DEVELOPMENTAL EXPOSURE ; HL-60 CELLS ; IN-VITRO ; NEONATAL EXPOSURE ; ADIPOSE-TISSUE ; RAT ; MOUSE ; DEATH ; ENVIRONMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000248467000003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8444]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Technol Ctr Hubei Entry Exit Inspect & Quarantine, Wuhan 430022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Xiao-Zhong,Xu, Ying,Hu, De-Cong,et al. Apoptosis induction on human hepatoma cells Hep G2 of decabrominated diphenyl ether (PBDE-209)[J]. TOXICOLOGY LETTERS,2007,171(1-2):19-28. |
| APA | Hu, Xiao-Zhong,Xu, Ying,Hu, De-Cong,Hui, Yang,&Yang, Fang-Xing.(2007).Apoptosis induction on human hepatoma cells Hep G2 of decabrominated diphenyl ether (PBDE-209).TOXICOLOGY LETTERS,171(1-2),19-28. |
| MLA | Hu, Xiao-Zhong,et al."Apoptosis induction on human hepatoma cells Hep G2 of decabrominated diphenyl ether (PBDE-209)".TOXICOLOGY LETTERS 171.1-2(2007):19-28. |
入库方式： OAI收割
来源：水生生物研究所
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