Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China
文献类型:期刊论文
作者 | Teng, Y.; Liu, H.; Lv, J. Q.; Fan, W. H.; Zhang, Q. Y.; Qin, Q. W. |
刊名 | ARCHIVES OF VIROLOGY |
出版日期 | 2007-08-01 |
卷号 | 152期号:8页码:1457-1465 |
ISSN号 | 0304-8608 |
关键词 | STRAND RNA VIRUSES NUCLEOTIDE-SEQUENCE GLYCOPROTEIN GENE FISH RHABDOVIRUS PROTEINS DOMAINS JUNCTIONS ALIGNMENT |
通讯作者 | Qin, QW, Sun Yat Sen Univ, Coll Life Sci, State Key Lab Biocontrol, 135 W Xingang Rd, Guangzhou 510275, Peoples R China |
中文摘要 | The complete genome of spring viraemia of carp virus (SVCV) strain A-1 isolated from cultured common carp (Cyprinus carpio) in China was sequenced and characterized. Reverse transcription-polymerase chain reaction (RT-PCR) derived clones were constructed and the DNA was sequenced. It showed that the entire genome of SVCV A-1 consists of 11,100 nucleotide base pairs, the predicted size of the viral RNA of rhabdoviruses. However, the additional insertions in bp 4633-4676 and bp 4684-4724 of SVCV A-1 were different from the other two published SVCV complete genomes. Five open reading frames (ORFs) of SVCV A-1 were identified and further confirmed by RT-PCR and DNA sequencing of their respective RT-PCR products. The 5 structural proteins encoded by the viral RNA were ordered 3'-N-P-M-G-L-5'. This is the first report of a complete genome sequence of SVCV isolated from cultured carp in China. Phylogenetic analysis indicates that SVCV A-1 is closely related to the members of the genus Vesiculovirus, family Rhabdoviridae. |
英文摘要 | The complete genome of spring viraemia of carp virus (SVCV) strain A-1 isolated from cultured common carp (Cyprinus carpio) in China was sequenced and characterized. Reverse transcription-polymerase chain reaction (RT-PCR) derived clones were constructed and the DNA was sequenced. It showed that the entire genome of SVCV A-1 consists of 11,100 nucleotide base pairs, the predicted size of the viral RNA of rhabdoviruses. However, the additional insertions in bp 4633-4676 and bp 4684-4724 of SVCV A-1 were different from the other two published SVCV complete genomes. Five open reading frames (ORFs) of SVCV A-1 were identified and further confirmed by RT-PCR and DNA sequencing of their respective RT-PCR products. The 5 structural proteins encoded by the viral RNA were ordered 3'-N-P-M-G-L-5'. This is the first report of a complete genome sequence of SVCV isolated from cultured carp in China. Phylogenetic analysis indicates that SVCV A-1 is closely related to the members of the genus Vesiculovirus, family Rhabdoviridae. |
学科主题 | Virology |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Virology |
研究领域[WOS] | Virology |
关键词[WOS] | STRAND RNA VIRUSES ; NUCLEOTIDE-SEQUENCE ; GLYCOPROTEIN GENE ; FISH ; RHABDOVIRUS ; PROTEINS ; DOMAINS ; JUNCTIONS ; ALIGNMENT |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000248374600004 |
公开日期 | 2010-10-13 |
源URL | [http://ir.ihb.ac.cn/handle/152342/8464] |
专题 | 水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文 |
作者单位 | 1.Sun Yat Sen Univ, Coll Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China 2.Shenzhen Exit & Entry Inspec & Quarantine Bur, Key Lab Aquat Anim Dis, Shenzhen, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China |
推荐引用方式 GB/T 7714 | Teng, Y.,Liu, H.,Lv, J. Q.,et al. Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China[J]. ARCHIVES OF VIROLOGY,2007,152(8):1457-1465. |
APA | Teng, Y.,Liu, H.,Lv, J. Q.,Fan, W. H.,Zhang, Q. Y.,&Qin, Q. W..(2007).Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China.ARCHIVES OF VIROLOGY,152(8),1457-1465. |
MLA | Teng, Y.,et al."Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China".ARCHIVES OF VIROLOGY 152.8(2007):1457-1465. |
入库方式: OAI收割
来源:水生生物研究所
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