中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China

文献类型:期刊论文

作者Teng, Y.; Liu, H.; Lv, J. Q.; Fan, W. H.; Zhang, Q. Y.; Qin, Q. W.
刊名ARCHIVES OF VIROLOGY
出版日期2007-08-01
卷号152期号:8页码:1457-1465
ISSN号0304-8608
关键词STRAND RNA VIRUSES NUCLEOTIDE-SEQUENCE GLYCOPROTEIN GENE FISH RHABDOVIRUS PROTEINS DOMAINS JUNCTIONS ALIGNMENT
通讯作者Qin, QW, Sun Yat Sen Univ, Coll Life Sci, State Key Lab Biocontrol, 135 W Xingang Rd, Guangzhou 510275, Peoples R China
中文摘要The complete genome of spring viraemia of carp virus (SVCV) strain A-1 isolated from cultured common carp (Cyprinus carpio) in China was sequenced and characterized. Reverse transcription-polymerase chain reaction (RT-PCR) derived clones were constructed and the DNA was sequenced. It showed that the entire genome of SVCV A-1 consists of 11,100 nucleotide base pairs, the predicted size of the viral RNA of rhabdoviruses. However, the additional insertions in bp 4633-4676 and bp 4684-4724 of SVCV A-1 were different from the other two published SVCV complete genomes. Five open reading frames (ORFs) of SVCV A-1 were identified and further confirmed by RT-PCR and DNA sequencing of their respective RT-PCR products. The 5 structural proteins encoded by the viral RNA were ordered 3'-N-P-M-G-L-5'. This is the first report of a complete genome sequence of SVCV isolated from cultured carp in China. Phylogenetic analysis indicates that SVCV A-1 is closely related to the members of the genus Vesiculovirus, family Rhabdoviridae.
英文摘要The complete genome of spring viraemia of carp virus (SVCV) strain A-1 isolated from cultured common carp (Cyprinus carpio) in China was sequenced and characterized. Reverse transcription-polymerase chain reaction (RT-PCR) derived clones were constructed and the DNA was sequenced. It showed that the entire genome of SVCV A-1 consists of 11,100 nucleotide base pairs, the predicted size of the viral RNA of rhabdoviruses. However, the additional insertions in bp 4633-4676 and bp 4684-4724 of SVCV A-1 were different from the other two published SVCV complete genomes. Five open reading frames (ORFs) of SVCV A-1 were identified and further confirmed by RT-PCR and DNA sequencing of their respective RT-PCR products. The 5 structural proteins encoded by the viral RNA were ordered 3'-N-P-M-G-L-5'. This is the first report of a complete genome sequence of SVCV isolated from cultured carp in China. Phylogenetic analysis indicates that SVCV A-1 is closely related to the members of the genus Vesiculovirus, family Rhabdoviridae.
学科主题Virology
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Virology
研究领域[WOS]Virology
关键词[WOS]STRAND RNA VIRUSES ; NUCLEOTIDE-SEQUENCE ; GLYCOPROTEIN GENE ; FISH ; RHABDOVIRUS ; PROTEINS ; DOMAINS ; JUNCTIONS ; ALIGNMENT
收录类别SCI
语种英语
WOS记录号WOS:000248374600004
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/8464]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位1.Sun Yat Sen Univ, Coll Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China
2.Shenzhen Exit & Entry Inspec & Quarantine Bur, Key Lab Aquat Anim Dis, Shenzhen, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
推荐引用方式
GB/T 7714
Teng, Y.,Liu, H.,Lv, J. Q.,et al. Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China[J]. ARCHIVES OF VIROLOGY,2007,152(8):1457-1465.
APA Teng, Y.,Liu, H.,Lv, J. Q.,Fan, W. H.,Zhang, Q. Y.,&Qin, Q. W..(2007).Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China.ARCHIVES OF VIROLOGY,152(8),1457-1465.
MLA Teng, Y.,et al."Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China".ARCHIVES OF VIROLOGY 152.8(2007):1457-1465.

入库方式: OAI收割

来源:水生生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。