Characterization of novel microsatellite loci in rare minnow (Gobiocypris rarus) and amplification in closely related species in Gobioninae
文献类型：期刊论文
| 作者 | Liao, Xiaolin; Wang, Dan; Yu, Xiaomu; Li, Weitao; Cheng, Lei; Wang, Jianwei; Tong, Jingou |
| 刊名 | CONSERVATION GENETICS
 |
| 出版日期 | 2007-08-01 |
| 卷号 | 8期号:4页码:1003-1007 |
| 关键词 | Gobiocypris rarus microsatellite genetic diversity cross-species amplification conservation genetics |
| ISSN号 | 1566-0621 |
| 通讯作者 | Liao, XL, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Rare minnow (Gobiocypris rarus) is an endangered small fish endemic to upper reach of the Yangtze River. From a (GT)n enriched genomic library, 32 microsatellites were isolated and characterized. Nineteen of these loci were polymorphic in a test population with alleles ranging from 2-7, and observed and expected heterozygosities from zero to 0.8438, and 0.2679 to 0.8264, respectively. In the cross-species amplifications, 13 out of 19 polymorphic loci were found to be also polymorphic in at least one of the 7 closely related species of the subfamily Gobioninae. These polymorphic microsatellite loci should provide sufficient level of genetic diversity to evaluate the fine-scale population structure in rare minnow and its closely related species for the conservation purpose. |
| 英文摘要 | Rare minnow (Gobiocypris rarus) is an endangered small fish endemic to upper reach of the Yangtze River. From a (GT)n enriched genomic library, 32 microsatellites were isolated and characterized. Nineteen of these loci were polymorphic in a test population with alleles ranging from 2-7, and observed and expected heterozygosities from zero to 0.8438, and 0.2679 to 0.8264, respectively. In the cross-species amplifications, 13 out of 19 polymorphic loci were found to be also polymorphic in at least one of the 7 closely related species of the subfamily Gobioninae. These polymorphic microsatellite loci should provide sufficient level of genetic diversity to evaluate the fine-scale population structure in rare minnow and its closely related species for the conservation purpose. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biodiversity Conservation; Genetics & Heredity |
| 类目[WOS] | Biodiversity Conservation ; Genetics & Heredity |
| 研究领域[WOS] | Biodiversity & Conservation ; Genetics & Heredity |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000248300300025 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8478]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liao, Xiaolin,Wang, Dan,Yu, Xiaomu,et al. Characterization of novel microsatellite loci in rare minnow (Gobiocypris rarus) and amplification in closely related species in Gobioninae[J]. CONSERVATION GENETICS,2007,8(4):1003-1007. |
| APA | Liao, Xiaolin.,Wang, Dan.,Yu, Xiaomu.,Li, Weitao.,Cheng, Lei.,...&Tong, Jingou.(2007).Characterization of novel microsatellite loci in rare minnow (Gobiocypris rarus) and amplification in closely related species in Gobioninae.CONSERVATION GENETICS,8(4),1003-1007. |
| MLA | Liao, Xiaolin,et al."Characterization of novel microsatellite loci in rare minnow (Gobiocypris rarus) and amplification in closely related species in Gobioninae".CONSERVATION GENETICS 8.4(2007):1003-1007. |
入库方式： OAI收割
来源：水生生物研究所
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