Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells
文献类型：期刊论文
| 作者 | Huang, You-Hua; Huang, Xiao-Hong; Gui, Jian-Fang; Zhang, Qi-Ya |
| 刊名 | APOPTOSIS
 |
| 出版日期 | 2007-09-01 |
| 卷号 | 12期号:9页码:1569-1577 |
| 关键词 | fish cell apoptosis mitochondrion mitochondrial fragmentation rana glylio virus (RGV) caspase |
| ISSN号 | 1360-8185 |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | A fish cell line, fathead minnow (FHM) cell, was used to investigate the alteration of mitochondrial dynamics and the mechanism of apoptosis under Rana grylio virus (RGV) infection. Microscopy observations, flow-cytometry analysis and molecular marker detection revealed the apoptotic fate of the RGV-infected cells. Some typical apoptotic characteristics, such as chromatin condensation, DNA fragmentation and mitochondrial fragmentation, were observed, and significantly morphological changes of mitochondria, including size, shape, internal structure and distribution, were revealed. The mitochondria in RGV-infected cells were aggregated around the viromatrix, and the aggregation could be blocked by colchicine. Moreover, the Delta psi m collapse was induced, and caspase-9 and caspase-3 were activated in the RGV-infected cells. In addition, NF-kappa B activation and intracellular Ca2+ increase were also detected at different times after infection. The data revealed the detailed dynamics of mitochondrion-mediated apoptosis induced by an iridovirus, and provided the first report on mitochondrial fragmentation during virus-induced apoptosis in fish cells. |
| 英文摘要 | A fish cell line, fathead minnow (FHM) cell, was used to investigate the alteration of mitochondrial dynamics and the mechanism of apoptosis under Rana grylio virus (RGV) infection. Microscopy observations, flow-cytometry analysis and molecular marker detection revealed the apoptotic fate of the RGV-infected cells. Some typical apoptotic characteristics, such as chromatin condensation, DNA fragmentation and mitochondrial fragmentation, were observed, and significantly morphological changes of mitochondria, including size, shape, internal structure and distribution, were revealed. The mitochondria in RGV-infected cells were aggregated around the viromatrix, and the aggregation could be blocked by colchicine. Moreover, the Delta psi m collapse was induced, and caspase-9 and caspase-3 were activated in the RGV-infected cells. In addition, NF-kappa B activation and intracellular Ca2+ increase were also detected at different times after infection. The data revealed the detailed dynamics of mitochondrion-mediated apoptosis induced by an iridovirus, and provided the first report on mitochondrial fragmentation during virus-induced apoptosis in fish cells. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | NF-KAPPA-B ; IN-VITRO ; DEATH ; FISSION ; INDUCTION ; NECROSIS ; FUSION ; LINE ; IRIDOVIRUS ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000248325200002 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8482]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, You-Hua,Huang, Xiao-Hong,Gui, Jian-Fang,et al. Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells[J]. APOPTOSIS,2007,12(9):1569-1577. |
| APA | Huang, You-Hua,Huang, Xiao-Hong,Gui, Jian-Fang,&Zhang, Qi-Ya.(2007).Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells.APOPTOSIS,12(9),1569-1577. |
| MLA | Huang, You-Hua,et al."Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells".APOPTOSIS 12.9(2007):1569-1577. |
入库方式： OAI收割
来源：水生生物研究所
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