A Novel MALDI Matrix for Analyzing Peptides and Proteins: Paraffin Wax Immobilized Matrix
文献类型：期刊论文
| 作者 | Wei YL(魏远隆) ; Mei Y(梅媛) ; Xu Z(许哲) ; Wang CH(王翠红) ; Guo YL(郭寅龙) ; Du YP(杜一平) ; Zhang WB(张维冰) |
| 刊名 | Chin. J. Chem.
 |
| 出版日期 | 2009 |
| 卷号 | 27期号:1页码:105-110 |
| ISSN号 | 1001-604X |
| 其他题名 | 新型石蜡固化基质在肽及蛋白MALDI分析中的应用 |
| 通讯作者 | 郭寅龙 ; 杜一平 |
| 英文摘要 | A new kind of MALDI matrix. termed paraffin wax immobilized matrix, was used to study peptide mixtures and proteins. During the preparation process, the paraffin wax was heated and coated on the stainless-steel tartlet plate. and then 2,5-dihydrobenzoic acid (DHB) was deposited on the paraffin layer and stainless-steel target plate to obtain different kinds of matrix spots. The morphology of matrices on different supports and peptide-matrix co-crystallization were observed by a high resolution digital-video microscopy system. Peptide mixtures and bovine serum albumin (BSA) digests were used to investigate the performance of the immobilized matrices on the paraffin target. The MALDI-FTMS analysis results also showed that the detection sensitivity of matrices immobilized in the paraffin sample support was better than that on other sample supports. |
| 学科主题 | 分析化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1002/cjoc.200990000 |
| 语种 | 英语 |
| WOS记录号 | WOS:000264039400018 |
| 公开日期 | 2013-02-25 |
| 源URL | [http://202.127.28.38/handle/331003/19988]  |
| 专题 | 上海有机化学研究所_分析化学研究室 |
| 推荐引用方式 GB/T 7714 | Wei YL,Mei Y,Xu Z,et al. A Novel MALDI Matrix for Analyzing Peptides and Proteins: Paraffin Wax Immobilized Matrix[J]. Chin. J. Chem.,2009,27(1):105-110. |
| APA | 魏远隆.,梅媛.,许哲.,王翠红.,郭寅龙.,...&张维冰.(2009).A Novel MALDI Matrix for Analyzing Peptides and Proteins: Paraffin Wax Immobilized Matrix.Chin. J. Chem.,27(1),105-110. |
| MLA | 魏远隆,et al."A Novel MALDI Matrix for Analyzing Peptides and Proteins: Paraffin Wax Immobilized Matrix".Chin. J. Chem. 27.1(2009):105-110. |
入库方式： OAI收割
来源：上海有机化学研究所
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