Determination of beta-Lactamase Residues in Milk Using Matrix-Assisted Laser Desorption/Ionization Fourier Transform Mass Spectrometry
文献类型：期刊论文
| 作者 | Xu Z(许哲) ; Wang HY(王昊阳) ; Huang SX(黄士新) ; Wei YL(魏远隆) ; Yao SJ(姚胜军) ; Guo YL(郭寅龙) |
| 刊名 | Anal. Chem.
 |
| 出版日期 | 2010 |
| 卷号 | 82期号:5页码:2113-2118 |
| ISSN号 | 0003-2700 |
| 其他题名 | 牛奶中β-内酰胺酶残留的基质辅助激光解吸/离子化傅立叶变换质谱法测定 |
| 通讯作者 | 郭寅龙 |
| 英文摘要 | A selective, fast, and effective enzyme assay based on matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) for quantifying beta-lactamase, an illegal additive in milk products, has been reported. The strengths of the mass spectrometric assay are its response to all substrate and products, simple and direct detection of the conversion of substrate, and facile determination of enzyme activity. Also, MALDI MS is tolerant to many buffer salts, and reagents without the requirement of complicated sample pretreatment procedures. In this study, the approach was used to detect the presence of beta-lactamases (BLA) in milk samples. ne amount of BLA that could be determined in it milk sample is 6 x 10(3) U.mL(-1) by this approach. To test the strategy, it has been applied to the fortified milk (adding a BLA product known as an antimicrobial destroyer). It is then tested whether the pasteurization procedure of the milk process affects the activity of BLA in milk samples. This study offers a perspective into the utility of MALDI-FTMS as an alternative detection tool for BLA screening in milk. |
| 学科主题 | 分析化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1021/ac9019945 |
| 语种 | 英语 |
| WOS记录号 | WOS:000274841300076 |
| 公开日期 | 2013-02-25 |
| 源URL | [http://202.127.28.38/handle/331003/20020]  |
| 专题 | 上海有机化学研究所_分析化学研究室 |
| 推荐引用方式 GB/T 7714 | Xu Z,Wang HY,Huang SX,et al. Determination of beta-Lactamase Residues in Milk Using Matrix-Assisted Laser Desorption/Ionization Fourier Transform Mass Spectrometry[J]. Anal. Chem.,2010,82(5):2113-2118. |
| APA | 许哲,王昊阳,黄士新,魏远隆,姚胜军,&郭寅龙.(2010).Determination of beta-Lactamase Residues in Milk Using Matrix-Assisted Laser Desorption/Ionization Fourier Transform Mass Spectrometry.Anal. Chem.,82(5),2113-2118. |
| MLA | 许哲,et al."Determination of beta-Lactamase Residues in Milk Using Matrix-Assisted Laser Desorption/Ionization Fourier Transform Mass Spectrometry".Anal. Chem. 82.5(2010):2113-2118. |
入库方式： OAI收割
来源：上海有机化学研究所
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