Integration of High Accuracy N-Terminus Identification in Peptide Sequencing and Comparative Protein Analysis Via Isothiocyanate-Based Isotope Labeling Reagent with ESI Ion-trap TOF MS
文献类型：期刊论文
| 作者 | Leng JP(冷嘉鹏) ; Wang HY(王昊阳) ; Zhang L(张立) ; Zhang J(张菁) ; Wang H(汪航) ; Cai TT(蔡婷婷) ; Yao JT(姚劲挺) ; Guo YL(郭寅龙) |
| 刊名 | J. Am. Soc. Mass Spectrom.
 |
| 出版日期 | 2011 |
| 卷号 | 22期号:7页码:1204-1213 |
| ISSN号 | 1044-0305 |
| 其他题名 | 应用异硫氰酸基同位素标记试剂结合电喷雾离子阱飞行时间质谱进行高准确性的多肽测序以及蛋白质定量分析 |
| 通讯作者 | 郭寅龙 |
| 英文摘要 | A multifunctional isothiocyanate-based isotope labeling reagent, [d (0)]-/[d (6)]-4,6-dimethoxy pyrimidine-2-isothiocyanate (DMPITC), has been developed for accurate N-terminus identification in peptide sequencing and comparative protein analysis by ESI Ion-trap TOF mass spectrometry. In contrast with the conventional labeling reagent phenyl isothiocyanate (PITC), DMPITC showed more desirable properties such as rapid labeling, sensitivity enhancement, and facilitating peptide sequencing. More significantly, DMPITC-based labeling strategy possessed the capacity of higher reliable N-terminus identification owning to the high-yield b(1) ion combined with the isotope validation of 6 Da. Meanwhile, it also showed potential in differentiating isomeric residues of leucine and isoleucine at N-terminus on the basis of the relative abundance ratios between the fragment ions of their respective b(1) ions. The strategy not only allows accurate interpretation for peptide but also ensures rapid and sensitive comparative analysis for protein by direct MS analysis. Using trypsin-digested bovine serum albumin (BSA), both peptide N-terminus identification and quantitative analysis were accomplished with high accuracy, efficiency, and reproducibility. The application of DMPITC-based labeling strategy is expected to serve as a promising tool for proteome research. |
| 学科主题 | 分析化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1007/s13361-011-0129-5 |
| 语种 | 英语 |
| WOS记录号 | WOS:000291257400011 |
| 公开日期 | 2013-02-25 |
| 源URL | [http://202.127.28.38/handle/331003/20036]  |
| 专题 | 上海有机化学研究所_分析化学研究室 |
| 推荐引用方式 GB/T 7714 | Leng JP,Wang HY,Zhang L,et al. Integration of High Accuracy N-Terminus Identification in Peptide Sequencing and Comparative Protein Analysis Via Isothiocyanate-Based Isotope Labeling Reagent with ESI Ion-trap TOF MS[J]. J. Am. Soc. Mass Spectrom.,2011,22(7):1204-1213. |
| APA | 冷嘉鹏.,王昊阳.,张立.,张菁.,汪航.,...&郭寅龙.(2011).Integration of High Accuracy N-Terminus Identification in Peptide Sequencing and Comparative Protein Analysis Via Isothiocyanate-Based Isotope Labeling Reagent with ESI Ion-trap TOF MS.J. Am. Soc. Mass Spectrom.,22(7),1204-1213. |
| MLA | 冷嘉鹏,et al."Integration of High Accuracy N-Terminus Identification in Peptide Sequencing and Comparative Protein Analysis Via Isothiocyanate-Based Isotope Labeling Reagent with ESI Ion-trap TOF MS".J. Am. Soc. Mass Spectrom. 22.7(2011):1204-1213. |
入库方式： OAI收割
来源：上海有机化学研究所
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