Polyphyllin D, a steroidal saponin from Paris polyphylla, inhibits endothelial cell functions in vitro and angiogenesis in zebrafish embryos in vivo
文献类型：期刊论文
| 作者 | Judy Yuet-Wa Chan ; Johnny Chi-Man Koon ; Xiaozhou Liu ; Michael Detmar ; Yu B(俞飚) ; Siu-Kai Kong ; Kwok-Pui Fung |
| 刊名 | J. Ethnopharmacol.
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| 出版日期 | 2011 |
| 卷号 | 137期号:1页码:64-69 |
| ISSN号 | 0378-8741 |
| 其他题名 | 甾体皂苷Polyphyllin D, 能体外抑制血管内皮细胞功能, 并能在体内一个斑马鱼胚胎血管生成 |
| 通讯作者 | Kwok-Pui Fung |
| 英文摘要 | Ethnopharmacological relevance: Angiogenesis, the process of blood vessel formation, is critical to tumour growth. The importance of angiogenesis in tumour development has lead to the development of antiangiogenic strategies to inhibit tumour growth. In this study, polyphyllin D (PD), an active component in Chinese herb, Paris polyphylla, was evaluated for its potential anti-angiogenic effects. Materials and methods: The inhibitory effects of PD on three important processes involved in angiogenesis, i.e. proliferation, migration and differentiation were examined using human microvascular endothelial cell line HMEC-1 by MTT assay, scratch assay and tube formation assay, respectively. Using zebrafish embryos as an animal model of angiogenesis, the anti-angiogenic effect of PD was further verified in vivo. Results: PD suppressed the growth of HMEC-1 cells at 0.1-0.4 mu M without toxic effects. At 0.3 mu M and 0.4 mu M, PD significantly inhibited endothelial cell migration and capillary tube formation. About 70% of the zebrafish embryos showed defects in intersegmental vessel formation upon treatment with PD at concentrations of 0.156 mu M and 0.313 mu M. Conclusion: The anti-angiogenic effects of PD have been explored in the study which implied a potential therapeutic development of PD in cancer treatment. (C) 2011 Elsevier Ireland Ltd. All rights reserved. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1016/j.jep.2011.04.021 |
| 语种 | 英语 |
| WOS记录号 | WOS:000295236700008 |
| 公开日期 | 2013-02-25 |
| 源URL | [http://202.127.28.38/handle/331003/19155]  |
| 专题 | 上海有机化学研究所_生命有机化学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Judy Yuet-Wa Chan,Johnny Chi-Man Koon,Xiaozhou Liu,et al. Polyphyllin D, a steroidal saponin from Paris polyphylla, inhibits endothelial cell functions in vitro and angiogenesis in zebrafish embryos in vivo[J]. J. Ethnopharmacol.,2011,137(1):64-69. |
| APA | Judy Yuet-Wa Chan.,Johnny Chi-Man Koon.,Xiaozhou Liu.,Michael Detmar.,俞飚.,...&Kwok-Pui Fung.(2011).Polyphyllin D, a steroidal saponin from Paris polyphylla, inhibits endothelial cell functions in vitro and angiogenesis in zebrafish embryos in vivo.J. Ethnopharmacol.,137(1),64-69. |
| MLA | Judy Yuet-Wa Chan,et al."Polyphyllin D, a steroidal saponin from Paris polyphylla, inhibits endothelial cell functions in vitro and angiogenesis in zebrafish embryos in vivo".J. Ethnopharmacol. 137.1(2011):64-69. |
入库方式： OAI收割
来源：上海有机化学研究所
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