Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara)
文献类型：期刊论文
| 作者 | Zhou, Guang-Zhou; Li, Zheng-Qiu; Yuan, Xiu-Ping; Zhang, Qi-Ya |
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 9期号:3页码:370-376 |
| 关键词 | fish cell line iridovirus red spotted grouper Epinephelus akaara rhabdovirus transfection |
| ISSN号 | 1436-2228 |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | A marine fish cell line from the snout of red spotted grouper Epinephelus akaara, a protogynous hermaphrodite, was established, characterized, and subcultured with more than 60 passages. The grouper snout cell line (GSC) cells multiplied well in Dulbecco's modified Eagle's medium (DMEM) medium supplemented with 10% fetal bovine serum. The optimal growth temperature was 25 degrees C, and morphologically the cells were fibroblastic. Chromosome analysis revealed that the GSC cell line has a normal diploid karyotype with 2n = 8st + 40t. A virus titration study indicated that the cells were susceptible to turbot Scophthalmus Maximus rhabdovirus (SMRV) (10(8.5) TCID50 ml(-1)), while the viral titer of frog Rana grylio virus 9807 (RGV(9807)) reached 10(3.5) TCID50 ml-1. The infection was confirmed by cytopathic effect (CPE), immunofluorescence, and electron microscopy experiments, which detected the viral particles in the cytoplasm of virus-infected cells, respectively. Further, significant fluorescent signals were observed when the GSC cells were transfected with pEGFP vector DNA, indicating their potential utility for transgenic and genetic manipulation studies. |
| 英文摘要 | A marine fish cell line from the snout of red spotted grouper Epinephelus akaara, a protogynous hermaphrodite, was established, characterized, and subcultured with more than 60 passages. The grouper snout cell line (GSC) cells multiplied well in Dulbecco's modified Eagle's medium (DMEM) medium supplemented with 10% fetal bovine serum. The optimal growth temperature was 25 degrees C, and morphologically the cells were fibroblastic. Chromosome analysis revealed that the GSC cell line has a normal diploid karyotype with 2n = 8st + 40t. A virus titration study indicated that the cells were susceptible to turbot Scophthalmus Maximus rhabdovirus (SMRV) (10(8.5) TCID50 ml(-1)), while the viral titer of frog Rana grylio virus 9807 (RGV(9807)) reached 10(3.5) TCID50 ml-1. The infection was confirmed by cytopathic effect (CPE), immunofluorescence, and electron microscopy experiments, which detected the viral particles in the cytoplasm of virus-infected cells, respectively. Further, significant fluorescent signals were observed when the GSC cells were transfected with pEGFP vector DNA, indicating their potential utility for transgenic and genetic manipulation studies. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biotechnology & Applied Microbiology; Marine & Freshwater Biology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 关键词[WOS] | RANA-GRYLIO VIRUS ; MOLECULAR-CLONING ; FISH ; COIOIDES ; RHABDOVIRUS ; IRIDOVIRUS ; EXPRESSION ; MANAGEMENT ; NODAVIRUS ; CHIMERAS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247207600008 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8550]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Guang-Zhou,Li, Zheng-Qiu,Yuan, Xiu-Ping,et al. Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara)[J]. MARINE BIOTECHNOLOGY,2007,9(3):370-376. |
| APA | Zhou, Guang-Zhou,Li, Zheng-Qiu,Yuan, Xiu-Ping,&Zhang, Qi-Ya.(2007).Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara).MARINE BIOTECHNOLOGY,9(3),370-376. |
| MLA | Zhou, Guang-Zhou,et al."Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara)".MARINE BIOTECHNOLOGY 9.3(2007):370-376. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。