Construction and characterization of a Lipotes vexillifer genomic DNA BAC library
文献类型：期刊论文
| 作者 | Du, Bo; Zhang, Xian-feng; Fang, Sheng-guo; Wang, Ding |
| 刊名 | ZOOLOGICAL SCIENCE
 |
| 出版日期 | 2007-04-01 |
| 卷号 | 24期号:4页码:401-407 |
| 关键词 | Lipotes vexillifer genomic DNA library alpha lactalbumin gene gene clone endangered species |
| ISSN号 | 0289-0003 |
| 通讯作者 | Wang, D, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | We constructed a genomic DNA library for Lipotes vexillifer (L. vexillifer), the Baiji or Yangtze River dolphin, one of the most endangered mammals in the world. The library consists of 149,000 BAC clones, with an average insert size of 83 kb, representing approximately 3.4 haploid genome equivalents. PCR amplification of four known L. vexillifer genes yielded two to four positive clones each. To demonstrate the utility of this library, we isolated and sequenced the L. vexillifer alpha lactalbumin gene, which is a gene specific to mammals and one which has been widely used as molecular tool in phylogenetic analysis. We also end-sequenced 20 randomly selected clones, resulting in the identification of at least five new L. vexilliter genes, five SSR loci, and one SINE locus. These results suggest that this library is a valuable resource for candidate gene cloning, physical mapping, and genome sequencing of this important and threatened species. |
| 英文摘要 | We constructed a genomic DNA library for Lipotes vexillifer (L. vexillifer), the Baiji or Yangtze River dolphin, one of the most endangered mammals in the world. The library consists of 149,000 BAC clones, with an average insert size of 83 kb, representing approximately 3.4 haploid genome equivalents. PCR amplification of four known L. vexillifer genes yielded two to four positive clones each. To demonstrate the utility of this library, we isolated and sequenced the L. vexillifer alpha lactalbumin gene, which is a gene specific to mammals and one which has been widely used as molecular tool in phylogenetic analysis. We also end-sequenced 20 randomly selected clones, resulting in the identification of at least five new L. vexilliter genes, five SSR loci, and one SINE locus. These results suggest that this library is a valuable resource for candidate gene cloning, physical mapping, and genome sequencing of this important and threatened species. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Zoology |
| 类目[WOS] | Zoology |
| 研究领域[WOS] | Zoology |
| 关键词[WOS] | ARTIFICIAL CHROMOSOME LIBRARY ; PLANT ARABIDOPSIS-THALIANA ; RESISTANCE GENE ; CETACEAN LINEAGES ; RIVER DOLPHINS ; SEQUENCE ; IDENTIFICATION ; WHALES ; MITOCHONDRIAL ; PROJECT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000246955600011 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8556]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Zhejiang Univ, Key Lab Conservat Genet & Reprod Biol Endangered, Educ Minist, Hangzhou 310029, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Bo,Zhang, Xian-feng,Fang, Sheng-guo,et al. Construction and characterization of a Lipotes vexillifer genomic DNA BAC library[J]. ZOOLOGICAL SCIENCE,2007,24(4):401-407. |
| APA | Du, Bo,Zhang, Xian-feng,Fang, Sheng-guo,&Wang, Ding.(2007).Construction and characterization of a Lipotes vexillifer genomic DNA BAC library.ZOOLOGICAL SCIENCE,24(4),401-407. |
| MLA | Du, Bo,et al."Construction and characterization of a Lipotes vexillifer genomic DNA BAC library".ZOOLOGICAL SCIENCE 24.4(2007):401-407. |
入库方式： OAI收割
来源：水生生物研究所
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