In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer
文献类型：期刊论文
| 作者 | Li FQ(李风庆) ; Hou BH(侯碧和) ; Chen L(陈磊) ; Yao ZJ(姚祝军) ; Hong GF(洪国藩) |
| 刊名 | Acta Biochim. Biophys. Sin.
 |
| 出版日期 | 2008 |
| 卷号 | 40期号:9页码:783-789 |
| ISSN号 | 1672-9145 |
| 其他题名 | FRET实验观察NodD和诱导信号分子柚皮素的体外相互作用 |
| 通讯作者 | 洪国藩 |
| 英文摘要 | At initial stages in the Rhizobium legume symbiosis, most Modulation genes are controlled by NodD protein and plant inducers. Some genetic studies and other reports have suggested that NodD may be activated by its direct interaction with plant inducers. However, there has been no molecular evidence of such an inducing interaction. In this paper, we used fluorescence resonance energy transfer technique to see whether such an interaction exists between NodD and its activator, naringenin, in vitro. The tetracysteine motif (Cys-Cys-Pro-Gly-Cys-Cys) was genetically inserted into NodD to label NodD with 4',5'-bis(1,3,2-dithioarsolan-2-yl) fluorescein (FlAsH). Naringenin was labeled with fluorescein by chemical linking. In the fluorescence resonance energy transfer experiments in vitro, the fluorescence intensity of one acceptor, NodD(90R6)-FlAsH, increased by 13%. This suggests that NodD may directly interact with inducer naringenin in vitro and that the reaction centre is likely near hinge region 1 of NodD. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1093/abbs/40.9.783 |
| 语种 | 英语 |
| WOS记录号 | WOS:000259676100003 |
| 公开日期 | 2013-02-26 |
| 源URL | [http://202.127.28.38/handle/331003/21111]  |
| 专题 | 上海有机化学研究所_生命有机化学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li FQ,Hou BH,Chen L,et al. In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer[J]. Acta Biochim. Biophys. Sin.,2008,40(9):783-789. |
| APA | 李风庆,侯碧和,陈磊,姚祝军,&洪国藩.(2008).In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer.Acta Biochim. Biophys. Sin.,40(9),783-789. |
| MLA | 李风庆,et al."In vitro observation of the molecular interaction between NodD and its inducer naringenin as monitored by fluorescence resonance energy transfer".Acta Biochim. Biophys. Sin. 40.9(2008):783-789. |
入库方式： OAI收割
来源：上海有机化学研究所
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