Fish ES cells and applications to biotechnology
文献类型：期刊论文
| 作者 | Alvarez, M. Carmen; Bejar, Julia; Chen, Songlin; Hong, Yunhan |
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 9期号:2页码:117-127 |
| 关键词 | chimeras ES cells fish transgenics |
| ISSN号 | 1436-2228 |
| 通讯作者 | Alvarez, MC, Univ Malaga, Fac Sci, Dept Cell Biol & Genet, E-29071 Malaga, Spain |
| 中文摘要 | ES cells provide a promising tool for the generation of transgenic animals with site-directed mutations. When ES cells colonize germ cells in chimeras, transgenic animals with modified phenotypes are generated and used either for functional genomics studies or for improving productivity in commercial settings. Althought the ES cell approach has been limited to, mice, there is strong interest for developing the technology in fish.. We describe the step-by-step procedure for developing ES cells in fish. Key aspects include avoiding cell differentiation, specific in vitro traits of pluripotency, and, most importantly, testing for production of chimeric animals as the main evidence of pluripotency. The entire process focuses on two model species, zebrafish and medaka, in which most work has been done. The achievements attained in these species, as well as their applicability to other commercial fish, are discussed. Because of the difficulties relating to germ line competence, mostly of long-term fish ES cells, alternative cell-based approaches such as primordial germ cells and nuclear transfer need to be considered. Although progress to date has been slow, there are promising achievements in homologous recombination and alternative avenues yet to be explored that can bring ES technology in fish to fruition. |
| 英文摘要 | ES cells provide a promising tool for the generation of transgenic animals with site-directed mutations. When ES cells colonize germ cells in chimeras, transgenic animals with modified phenotypes are generated and used either for functional genomics studies or for improving productivity in commercial settings. Althought the ES cell approach has been limited to, mice, there is strong interest for developing the technology in fish.. We describe the step-by-step procedure for developing ES cells in fish. Key aspects include avoiding cell differentiation, specific in vitro traits of pluripotency, and, most importantly, testing for production of chimeric animals as the main evidence of pluripotency. The entire process focuses on two model species, zebrafish and medaka, in which most work has been done. The achievements attained in these species, as well as their applicability to other commercial fish, are discussed. Because of the difficulties relating to germ line competence, mostly of long-term fish ES cells, alternative cell-based approaches such as primordial germ cells and nuclear transfer need to be considered. Although progress to date has been slow, there are promising achievements in homologous recombination and alternative avenues yet to be explored that can bring ES technology in fish to fruition. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biotechnology & Applied Microbiology; Marine & Freshwater Biology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 关键词[WOS] | EMBRYONIC STEM-CELLS ; MEDAKA ORYZIAS-LATIPES ; GERM-LINE CHIMERAS ; GREEN FLUORESCENT PROTEIN ; EARLY ZEBRAFISH EMBRYOS ; VASA-LIKE GENE ; IN-VITRO ; TELOMERASE ACTIVITY ; HOMOLOGOUS RECOMBINATION ; HUMAN BLASTOCYSTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245949800001 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8612]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Univ Malaga, Fac Sci, Dept Cell Biol & Genet, E-29071 Malaga, Spain 2.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 4.Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore |
| 推荐引用方式 GB/T 7714 | Alvarez, M. Carmen,Bejar, Julia,Chen, Songlin,et al. Fish ES cells and applications to biotechnology[J]. MARINE BIOTECHNOLOGY,2007,9(2):117-127. |
| APA | Alvarez, M. Carmen,Bejar, Julia,Chen, Songlin,&Hong, Yunhan.(2007).Fish ES cells and applications to biotechnology.MARINE BIOTECHNOLOGY,9(2),117-127. |
| MLA | Alvarez, M. Carmen,et al."Fish ES cells and applications to biotechnology".MARINE BIOTECHNOLOGY 9.2(2007):117-127. |
入库方式： OAI收割
来源：水生生物研究所
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