Cloning, characterization and promoter analysis of common carp hairy/Enhancer-of-split-related gene, her6
文献类型：期刊论文
| 作者 | Liu, Jing; Sun, Yong-Hua; Wang, Na; Wang, Ya-Ping; Zhu, Zuo-Yan |
| 刊名 | JOURNAL OF GENETICS
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 85期号:3页码:171-178 |
| ISSN号 | 0022-1333 |
| 关键词 | her6 common carp promoter genomic walking expression pattern development |
| 通讯作者 | Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Some members of hairy/Enhancer-of-split-related gene (HES) family have important effects on axial mesoderm segmentation and the establishment and maintenance of the somite fringe. In fishes. the her6 gene, a member of the HES family, is the homologue Of heS1 in mammals and chicken. In this study, the her6 gene and its full-length cDNA from the common carp (Cyprinus carpio) were isolated and characterized. The genomic sequence of common carp her6 is approximately 1.7 kb. with four exons and three introns, and the full-length cDNA of 1314 bp encodes a Putative polypeptide of 271 amino acids. To analyse the promoter sequence of common carp her6. sequences of various lengths upstream from the transcription initiation site of her6 were fused to enhanced green fluorescent. protein gene (eGFP) and introduced into zebrafish embryos by microinjection to generate transgenic embryos. Our results show that the upstream sequence of 500 bp can direct highly efficient and tissue-specific expression of eGFP in zebrafish embryos. whereas a fragment of 200 bp containing the TATA box and a partial suppressor of hairless paired site sequence (SPS) is not sufficient to drive eGFP expression in zebrafish embryos. |
| 英文摘要 | Some members of hairy/Enhancer-of-split-related gene (HES) family have important effects on axial mesoderm segmentation and the establishment and maintenance of the somite fringe. In fishes. the her6 gene, a member of the HES family, is the homologue Of heS1 in mammals and chicken. In this study, the her6 gene and its full-length cDNA from the common carp (Cyprinus carpio) were isolated and characterized. The genomic sequence of common carp her6 is approximately 1.7 kb. with four exons and three introns, and the full-length cDNA of 1314 bp encodes a Putative polypeptide of 271 amino acids. To analyse the promoter sequence of common carp her6. sequences of various lengths upstream from the transcription initiation site of her6 were fused to enhanced green fluorescent. protein gene (eGFP) and introduced into zebrafish embryos by microinjection to generate transgenic embryos. Our results show that the upstream sequence of 500 bp can direct highly efficient and tissue-specific expression of eGFP in zebrafish embryos. whereas a fragment of 200 bp containing the TATA box and a partial suppressor of hairless paired site sequence (SPS) is not sufficient to drive eGFP expression in zebrafish embryos. |
| 学科主题 | Genetics & Heredity |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Genetics & Heredity |
| 研究领域[WOS] | Genetics & Heredity |
| 关键词[WOS] | MEDAKA ORYZIAS-LATIPES ; GFP REPORTER GENE ; PRESOMITIC MESODERM ; VERTEBRATE SEGMENTATION ; SOMITE SEGMENTATION ; DYNAMIC EXPRESSION ; ZEBRAFISH ; NOTCH ; HAIRY ; SOMITOGENESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245879500003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8614]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jing,Sun, Yong-Hua,Wang, Na,et al. Cloning, characterization and promoter analysis of common carp hairy/Enhancer-of-split-related gene, her6[J]. JOURNAL OF GENETICS,2006,85(3):171-178. |
| APA | Liu, Jing,Sun, Yong-Hua,Wang, Na,Wang, Ya-Ping,&Zhu, Zuo-Yan.(2006).Cloning, characterization and promoter analysis of common carp hairy/Enhancer-of-split-related gene, her6.JOURNAL OF GENETICS,85(3),171-178. |
| MLA | Liu, Jing,et al."Cloning, characterization and promoter analysis of common carp hairy/Enhancer-of-split-related gene, her6".JOURNAL OF GENETICS 85.3(2006):171-178. |
入库方式： OAI收割
来源：水生生物研究所
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