热门
Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus)
文献类型：期刊论文
| 作者 | Liu, Chunsheng; Yu, Ke; Shi, Xiongjie; Wang, Jingxian; Lam, Paul K. S.; Wu, Rudolf S. S.; Zhou, Bingsheng |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2007-05-01 |
| 卷号 | 82期号:2页码:135-143 |
| ISSN号 | 0166-445X |
| 关键词 | perfluorinated organic compounds primary cultured hepatocytes reactive oxygen species oxidative stress caspases apoptosis tilapia |
| 通讯作者 | Zhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Perfluorinated organic compounds (PFOCs) are emerging persistent organic pollutants (POPs) widely present in the environment, wildlife and human. We studied the cellular toxicology of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) on oxidative stress and induction of apoptosis in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Cultured hepatocytes were exposed to PFOS or PFOA (0, 1, 5, 15 and 30 mg L-1) for 24 h, and a dose-dependent decrease in cell viability was determined using trypan blue exclusion method. Significant induction of reactive oxygen species (ROS) accompanied by increases in activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were found, while activities of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were decreased. Glutathione (GSH) content was reduced following treatment of PFOA and PFOS. A dose-dependent increase in the lipid peroxidation (LPO) level (measured as maleic dialdehyde, MDA) was observed only in the PFOA exposure groups, whereas LPO remained unchanged in the PFOS exposure groups. Furthermore, a significant activation of caspase-3, -8, -9 activities was evident in both PFOS and PFOA exposure groups. Typical DNA fragmentation (DNA laddering) was further characterized by agarose gel electrophoresis. The overall results demonstrated that PFOS and PFOA are able to produce oxidative stress and induce apoptosis with involvement of caspases in primary cultured tilapia hepatocytes. (c) 2007 Elsevier B.V. All rights reserved. |
| 英文摘要 | Perfluorinated organic compounds (PFOCs) are emerging persistent organic pollutants (POPs) widely present in the environment, wildlife and human. We studied the cellular toxicology of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) on oxidative stress and induction of apoptosis in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Cultured hepatocytes were exposed to PFOS or PFOA (0, 1, 5, 15 and 30 mg L-1) for 24 h, and a dose-dependent decrease in cell viability was determined using trypan blue exclusion method. Significant induction of reactive oxygen species (ROS) accompanied by increases in activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were found, while activities of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were decreased. Glutathione (GSH) content was reduced following treatment of PFOA and PFOS. A dose-dependent increase in the lipid peroxidation (LPO) level (measured as maleic dialdehyde, MDA) was observed only in the PFOA exposure groups, whereas LPO remained unchanged in the PFOS exposure groups. Furthermore, a significant activation of caspase-3, -8, -9 activities was evident in both PFOS and PFOA exposure groups. Typical DNA fragmentation (DNA laddering) was further characterized by agarose gel electrophoresis. The overall results demonstrated that PFOS and PFOA are able to produce oxidative stress and induce apoptosis with involvement of caspases in primary cultured tilapia hepatocytes. (c) 2007 Elsevier B.V. All rights reserved. |
| 学科主题 | Marine & Freshwater Biology; Toxicology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | PERFLUOROOCTANE SULFONIC-ACID ; OXYGEN SPECIES PRODUCTION ; CARP CYPRINUS-CARPIO ; IN-VITRO ; PERFLUORINATED COMPOUNDS ; RAT-LIVER ; SERUM CONCENTRATIONS ; GENE-EXPRESSION ; FLUOROCHEMICALS ; EXPOSURE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245962400006 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8616]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Chunsheng,Yu, Ke,Shi, Xiongjie,et al. Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus)[J]. AQUATIC TOXICOLOGY,2007,82(2):135-143. |
| APA | Liu, Chunsheng.,Yu, Ke.,Shi, Xiongjie.,Wang, Jingxian.,Lam, Paul K. S..,...&Zhou, Bingsheng.(2007).Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus).AQUATIC TOXICOLOGY,82(2),135-143. |
| MLA | Liu, Chunsheng,et al."Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus)".AQUATIC TOXICOLOGY 82.2(2007):135-143. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。