Subcellular localization and characterization of G protein-coupled receptor homolog from lymphocystis disease virus isolated in China
文献类型：期刊论文
| 作者 | Huang, Youhua; Huang, Xiaohong; Zhang, Jing; Gui, Jianfang; Zhang, Qiya |
| 刊名 | VIRAL IMMUNOLOGY
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 20期号:1页码:150-159 |
| 关键词 | SARCOMA-ASSOCIATED-HERPESVIRUS KAPOSIS-SARCOMA CHEMOKINE RECEPTORS CYTOMEGALOVIRUS SEQUENCE ORF74 IRIDOVIRUS WITHDRAWAL APOPTOSIS SYSTEM |
| ISSN号 | 0882-8245 |
| 通讯作者 | Zhang, QY, Grad Univ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China |
| 中文摘要 | G protein-coupled receptors (GPCRs) constitute a large superfamily involved in various types of signal transduction pathways, and play an important role in coordinating the activation and migration of leukocytes to sites of infection and inflammation. Viral GPCRs, on the other hand, can help the virus to escape from host immune surveillance and contribute to viral pathogenesis. Lymphocystis disease virus isolated in China (LCDV-C) contains a putative homolog of cellular GPCRs, LCDV-C GPCR. In this paper, LCDV-C GPCR was cloned, and the subcellular localization and characterization of GPCR protein were investigated in fish cells. LCDV-C GPCR encoded a 325-amino acid peptide, containing a typical seven-transmembrane domain characteristic of the chemokine receptors and a conserved DRY motif that is usually essential for receptor activation. Transient transfection of GPCR-EGFP in fathead minnow (FHM) cells and epithelioma papulosum cyprini (EPC) cells indicated that LCDV-C GPCR was expressed abundantly in both the cytoplasm and nucleoplasm. Transient overexpression of GPCR in these two cells cannot induce obvious apoptosis. FHM cells stably expressing GPCR showed enhanced cell proliferation and significant anchorage-independent growth. The effects of GPCR protein on external apoptotic stimuli were examined. Few apoptotic bodies were observed in cells expressing GPCR treated with actinomycin D (ActD). Quantitative analysis of apoptotic cells indicated that a considerable decrease in the apoptotic fraction of cells expressing GPCR, compared with. the control cells, was detected after exposure to ActD and cycloheximide. These data suggest that LCDV-C GPCR may inhibit apoptosis as part of its potential mechanism in mediating cellular transformation. |
| 英文摘要 | G protein-coupled receptors (GPCRs) constitute a large superfamily involved in various types of signal transduction pathways, and play an important role in coordinating the activation and migration of leukocytes to sites of infection and inflammation. Viral GPCRs, on the other hand, can help the virus to escape from host immune surveillance and contribute to viral pathogenesis. Lymphocystis disease virus isolated in China (LCDV-C) contains a putative homolog of cellular GPCRs, LCDV-C GPCR. In this paper, LCDV-C GPCR was cloned, and the subcellular localization and characterization of GPCR protein were investigated in fish cells. LCDV-C GPCR encoded a 325-amino acid peptide, containing a typical seven-transmembrane domain characteristic of the chemokine receptors and a conserved DRY motif that is usually essential for receptor activation. Transient transfection of GPCR-EGFP in fathead minnow (FHM) cells and epithelioma papulosum cyprini (EPC) cells indicated that LCDV-C GPCR was expressed abundantly in both the cytoplasm and nucleoplasm. Transient overexpression of GPCR in these two cells cannot induce obvious apoptosis. FHM cells stably expressing GPCR showed enhanced cell proliferation and significant anchorage-independent growth. The effects of GPCR protein on external apoptotic stimuli were examined. Few apoptotic bodies were observed in cells expressing GPCR treated with actinomycin D (ActD). Quantitative analysis of apoptotic cells indicated that a considerable decrease in the apoptotic fraction of cells expressing GPCR, compared with. the control cells, was detected after exposure to ActD and cycloheximide. These data suggest that LCDV-C GPCR may inhibit apoptosis as part of its potential mechanism in mediating cellular transformation. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology; Virology |
| 类目[WOS] | Immunology ; Virology |
| 研究领域[WOS] | Immunology ; Virology |
| 关键词[WOS] | SARCOMA-ASSOCIATED-HERPESVIRUS ; KAPOSIS-SARCOMA ; CHEMOKINE RECEPTORS ; CYTOMEGALOVIRUS ; SEQUENCE ; ORF74 ; IRIDOVIRUS ; WITHDRAWAL ; APOPTOSIS ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245710300015 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8618]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Grad Univ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Youhua,Huang, Xiaohong,Zhang, Jing,et al. Subcellular localization and characterization of G protein-coupled receptor homolog from lymphocystis disease virus isolated in China[J]. VIRAL IMMUNOLOGY,2007,20(1):150-159. |
| APA | Huang, Youhua,Huang, Xiaohong,Zhang, Jing,Gui, Jianfang,&Zhang, Qiya.(2007).Subcellular localization and characterization of G protein-coupled receptor homolog from lymphocystis disease virus isolated in China.VIRAL IMMUNOLOGY,20(1),150-159. |
| MLA | Huang, Youhua,et al."Subcellular localization and characterization of G protein-coupled receptor homolog from lymphocystis disease virus isolated in China".VIRAL IMMUNOLOGY 20.1(2007):150-159. |
入库方式： OAI收割
来源：水生生物研究所
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