Mutations stabilize small subunit ribosomal RNA in desiccation-tolerant cyanobacteria Nostoc
文献类型：期刊论文
| 作者 | Han, D.; Hu, Z. |
| 刊名 | CURRENT MICROBIOLOGY
 |
| 出版日期 | 2007-04-01 |
| 卷号 | 54期号:4页码:254-259 |
| ISSN号 | 0343-8651 |
| 关键词 | OPTIMAL-GROWTH TEMPERATURES SECONDARY STRUCTURE COMMUNE CYANOBACTERIA SEQUENCE ALIGNMENT MESSENGER-RNA GENOMIC DNA PROKARYOTES SUBSTITUTION CONSTRAINTS PREDICTION |
| 通讯作者 | Hu, Z, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | The ribosomal RNA molecule is an ideal model for evaluating the stability of a gene product under desiccation stress. We isolated 8 Nostoc strains that had the capacity to withstand desiccation in habitats and sequenced their 16S rRNA genes. The stabilities of 16S rRNAs secondary structures, indicated by free energy change of folding, were compared among Nostoc and other related species. The results suggested that 163 rRNA secondary structures of the desiccation-tolerant Nostoc strains were more stable than that of planktonic Nostocaceae species. The stabilizing mutations were divided into two categories: (1) those causing GC to replace other types of base pairs in stems and (2) those causing extension of stems. By mapping stabilizing mutations onto the Nostoc phylogenetic tree based on 16S rRNA gene, it was shown that most of stabilizing mutations had evolved during adaptive radiation among Nostoc spp. The evolution of 16S rRNA along the Nostoc lineage is suggested to be selectively advantageous under desiccation stress. |
| 英文摘要 | The ribosomal RNA molecule is an ideal model for evaluating the stability of a gene product under desiccation stress. We isolated 8 Nostoc strains that had the capacity to withstand desiccation in habitats and sequenced their 16S rRNA genes. The stabilities of 16S rRNAs secondary structures, indicated by free energy change of folding, were compared among Nostoc and other related species. The results suggested that 163 rRNA secondary structures of the desiccation-tolerant Nostoc strains were more stable than that of planktonic Nostocaceae species. The stabilizing mutations were divided into two categories: (1) those causing GC to replace other types of base pairs in stems and (2) those causing extension of stems. By mapping stabilizing mutations onto the Nostoc phylogenetic tree based on 16S rRNA gene, it was shown that most of stabilizing mutations had evolved during adaptive radiation among Nostoc spp. The evolution of 16S rRNA along the Nostoc lineage is suggested to be selectively advantageous under desiccation stress. |
| 学科主题 | Microbiology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | OPTIMAL-GROWTH TEMPERATURES ; SECONDARY STRUCTURE ; COMMUNE CYANOBACTERIA ; SEQUENCE ALIGNMENT ; MESSENGER-RNA ; GENOMIC DNA ; PROKARYOTES ; SUBSTITUTION ; CONSTRAINTS ; PREDICTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245505700002 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8644]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, D.,Hu, Z.. Mutations stabilize small subunit ribosomal RNA in desiccation-tolerant cyanobacteria Nostoc[J]. CURRENT MICROBIOLOGY,2007,54(4):254-259. |
| APA | Han, D.,&Hu, Z..(2007).Mutations stabilize small subunit ribosomal RNA in desiccation-tolerant cyanobacteria Nostoc.CURRENT MICROBIOLOGY,54(4),254-259. |
| MLA | Han, D.,et al."Mutations stabilize small subunit ribosomal RNA in desiccation-tolerant cyanobacteria Nostoc".CURRENT MICROBIOLOGY 54.4(2007):254-259. |
入库方式： OAI收割
来源：水生生物研究所
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