Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi
文献类型：期刊论文
| 作者 | Sun, B. J.; Chang, M. X.; Song, Y.; Yao, W. J.; Nie, P. |
| 刊名 | VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
 |
| 出版日期 | 2007-03-15 |
| 卷号 | 116期号:1-2页码:26-36 |
| 关键词 | interferon regulatory factor IRF-1 IRF-7 gene organization real-time PCR mandarin fish Chinese perch Siniperca chuatsi |
| ISSN号 | 0165-2427 |
| 通讯作者 | Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | The genes of IRF-1 and IRF-7 have been cloned from the mandarin fish (Siniperca chuatsi). The IRF-1 gene has 4919 nucleotides (nt) and contains 10exons and 9introns, with an open reading frame (ORF) of 903 ntencoding301 aa. The IRF-7 gene has 6057 nt and also contains 10exons and 9introns, with an ORF of 1308 nt encoding 436 aa. The IRF-1 and IRF-7 genes have only one copy each in the genome. The transcription of IRF-1 and IRF-7 in different organs was analyzed by real-time PCR, and both molecules were constitutively expressed. The IRF-I and IRF-7 mRNAs were abundant in gill, spleen, kidney and pronephros. The temporal transcriptional changes for IRF-1, IRF-7 and Mx were investigated within 48 h after poly I: C stimulation in liver, gill, spleen and pronephros. An increased transcription was detected for IRF-1 and IRF-7 12 h post-stimulation, being earlier than the transcription of Mx protein; however, IRF-1 and IRF-7 transcription decreased while the Mx protein was stable at 48 h post-stimulation. (c) 2007 Published by Elsevier B.V. |
| 英文摘要 | The genes of IRF-1 and IRF-7 have been cloned from the mandarin fish (Siniperca chuatsi). The IRF-1 gene has 4919 nucleotides (nt) and contains 10exons and 9introns, with an open reading frame (ORF) of 903 ntencoding301 aa. The IRF-7 gene has 6057 nt and also contains 10exons and 9introns, with an ORF of 1308 nt encoding 436 aa. The IRF-1 and IRF-7 genes have only one copy each in the genome. The transcription of IRF-1 and IRF-7 in different organs was analyzed by real-time PCR, and both molecules were constitutively expressed. The IRF-I and IRF-7 mRNAs were abundant in gill, spleen, kidney and pronephros. The temporal transcriptional changes for IRF-1, IRF-7 and Mx were investigated within 48 h after poly I: C stimulation in liver, gill, spleen and pronephros. An increased transcription was detected for IRF-1 and IRF-7 12 h post-stimulation, being earlier than the transcription of Mx protein; however, IRF-1 and IRF-7 transcription decreased while the Mx protein was stable at 48 h post-stimulation. (c) 2007 Published by Elsevier B.V. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology; Veterinary Sciences |
| 类目[WOS] | Immunology ; Veterinary Sciences |
| 研究领域[WOS] | Immunology ; Veterinary Sciences |
| 关键词[WOS] | INTERFERON REGULATORY FACTOR ; PANCREATIC NECROSIS VIRUS ; TROUT ONCORHYNCHUS-MYKISS ; DOUBLE-STRANDED-RNA ; MOLECULAR-CLONING ; EXPRESSION ; ACTIVATION ; INDUCTION ; FACTOR-7 ; MX |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245259300003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8652]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, B. J.,Chang, M. X.,Song, Y.,et al. Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2007,116(1-2):26-36. |
| APA | Sun, B. J.,Chang, M. X.,Song, Y.,Yao, W. J.,&Nie, P..(2007).Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,116(1-2),26-36. |
| MLA | Sun, B. J.,et al."Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 116.1-2(2007):26-36. |
入库方式： OAI收割
来源：水生生物研究所
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