Isolation and characterization of Scophthalmus maximus rhabdovirus
文献类型：期刊论文
| 作者 | Zhang, Qi-Ya; Tao, Jian-Jun; Gui, Lang; Zhou, Guang-Zhou; Ruan, Hong-Mei; Li, Zhen-Qiu; Gui, Jian-Fang |
| 刊名 | DISEASES OF AQUATIC ORGANISMS
 |
| 出版日期 | 2007-02-28 |
| 卷号 | 74期号:2页码:95-105 |
| 关键词 | rhabdovirus Scophthalmus maximus fish viral disease rhabdovirus polymerase |
| ISSN号 | 0177-5103 |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | A rhabdovirus associated with a lethal hemorrhagic disease in cultured turbot Scophthalm us maximus Linnaeus was isolated. The virus induced typical cytopathogenic effects (CPE) in 9 of 15 fish cell lines examined and was then propagated and isolated from infected carp leucocyte cells (CLC). Electron microscopy observations revealed that the negatively stained virions had a typical bullet-shaped morphology with one rounded end and one flat base end. The bullet-shaped morphology was more obvious and clear in ultrathin sections of infected cells. Experimental infections also indicated that the S. maximus rhabdovirus (SMRV) was not only a viral pathogen for cultured turbot, but also had the ability to infect other fish species, such as freshwater grass carp. A partial nucleotide sequence of the SMRV polymerase gene was determined by RT-PCR using 2 pairs of degenerate primers designed according to the conserved sequences of rhabdovirus polymerase genes. Homology analysis, amino acid sequence alignment, and phylogenetic relationship analysis of the partial SMRV polymerase sequence indicated that SMRV was genetically distinct from other rhabdoviruses. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the purified SMRV revealed 5 major structural proteins, and their molecular masses were estimated to be about 250, 58, 47, 42, and 28 kDa. Significant serological reactivity differences were also observed between SMRV and its nearest neighbor, spring viremia of carp virus (SVCV). The data suggest that SMRV is likely a novel fish rhabdovirus, although it is closely related to rhabdoviruses in the genus Vesiculovirus. |
| 英文摘要 | A rhabdovirus associated with a lethal hemorrhagic disease in cultured turbot Scophthalm us maximus Linnaeus was isolated. The virus induced typical cytopathogenic effects (CPE) in 9 of 15 fish cell lines examined and was then propagated and isolated from infected carp leucocyte cells (CLC). Electron microscopy observations revealed that the negatively stained virions had a typical bullet-shaped morphology with one rounded end and one flat base end. The bullet-shaped morphology was more obvious and clear in ultrathin sections of infected cells. Experimental infections also indicated that the S. maximus rhabdovirus (SMRV) was not only a viral pathogen for cultured turbot, but also had the ability to infect other fish species, such as freshwater grass carp. A partial nucleotide sequence of the SMRV polymerase gene was determined by RT-PCR using 2 pairs of degenerate primers designed according to the conserved sequences of rhabdovirus polymerase genes. Homology analysis, amino acid sequence alignment, and phylogenetic relationship analysis of the partial SMRV polymerase sequence indicated that SMRV was genetically distinct from other rhabdoviruses. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the purified SMRV revealed 5 major structural proteins, and their molecular masses were estimated to be about 250, 58, 47, 42, and 28 kDa. Significant serological reactivity differences were also observed between SMRV and its nearest neighbor, spring viremia of carp virus (SVCV). The data suggest that SMRV is likely a novel fish rhabdovirus, although it is closely related to rhabdoviruses in the genus Vesiculovirus. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Veterinary Sciences |
| 关键词[WOS] | HEMORRHAGIC SEPTICEMIA VIRUS ; COMPLETE GENOMIC SEQUENCE ; FLOUNDER PARALICHTHYS-OLIVACEUS ; RANA-GRYLIO VIRUS ; POLYMERASE L GENE ; FISH RHABDOVIRUS ; SPRING VIREMIA ; CELL-LINE ; CARP ; VESICULOVIRUSES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245070700002 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8660]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qi-Ya,Tao, Jian-Jun,Gui, Lang,et al. Isolation and characterization of Scophthalmus maximus rhabdovirus[J]. DISEASES OF AQUATIC ORGANISMS,2007,74(2):95-105. |
| APA | Zhang, Qi-Ya.,Tao, Jian-Jun.,Gui, Lang.,Zhou, Guang-Zhou.,Ruan, Hong-Mei.,...&Gui, Jian-Fang.(2007).Isolation and characterization of Scophthalmus maximus rhabdovirus.DISEASES OF AQUATIC ORGANISMS,74(2),95-105. |
| MLA | Zhang, Qi-Ya,et al."Isolation and characterization of Scophthalmus maximus rhabdovirus".DISEASES OF AQUATIC ORGANISMS 74.2(2007):95-105. |
入库方式： OAI收割
来源：水生生物研究所
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