The recombinant beta subunit of C-phycocyanin inhibits cell proliferation and induces apoptosis
文献类型：期刊论文
| 作者 | Wang, Haizhen; Liu, Yongding; Gao, Xueliang; Carter, Christie L.; Liu, Zhi-Ren |
| 刊名 | CANCER LETTERS
 |
| 出版日期 | 2007-03-08 |
| 卷号 | 247期号:1页码:150-158 |
| 关键词 | beta-subunit of C-phycocyanin apoptosis cell cycle proliferation caspase beta-tubulin GAPDH |
| ISSN号 | 0304-3835 |
| 通讯作者 | Liu, ZR, Georgia State Univ, Georgia Canc Ctr, Dept Biol, Univ Plaza, Atlanta, GA 30303 USA |
| 中文摘要 | C-Phycocyanin (C-PC) from blue-green algae has been reported to have various pharmacological characteristics, including antiinflammatory and anti-tumor activities. In this study, we expressed the beta-subunit of C-PC (ref to as C-POP) in Escherichia coli. We found that the recombinant C-PC/beta has anti-cancer properties. Under the treatment of 5 mu M of the recombinant C-PC/beta, four different cancer cell lines accrued high proliferation inhibition and apoptotic induction. Substantially, a lower response occurred in non-cancer cells. We investigated the mechanism by which C-PC/beta inhibits cancer cell proliferation and induces apoptosis. We found that the C-PC/beta interacts with membrane-associated beta-tubulin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Under the treatment of the C-PC/beta, depolymerization of microtubules and actin-filaments were observed. The cells underwent apoptosis with an increase in caspase-3, and caspase-8 activities. The cell cycle was arrested at the G0/G1 phase under the treatment of C-PC/beta. In addition, the nuclear level of GAPDH decreased significantly. Decrease in the nuclear level of GAPDH prevents the cell cycle from entering into the S phase. Inhibition of cancer cell proliferation and induction of apoptosis may potentate the C-POP as a promising cancer prevention or therapy agent. (c) 2006 Elsevier Ireland Ltd. All rights reserved. |
| 英文摘要 | C-Phycocyanin (C-PC) from blue-green algae has been reported to have various pharmacological characteristics, including antiinflammatory and anti-tumor activities. In this study, we expressed the beta-subunit of C-PC (ref to as C-POP) in Escherichia coli. We found that the recombinant C-PC/beta has anti-cancer properties. Under the treatment of 5 mu M of the recombinant C-PC/beta, four different cancer cell lines accrued high proliferation inhibition and apoptotic induction. Substantially, a lower response occurred in non-cancer cells. We investigated the mechanism by which C-PC/beta inhibits cancer cell proliferation and induces apoptosis. We found that the C-PC/beta interacts with membrane-associated beta-tubulin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Under the treatment of the C-PC/beta, depolymerization of microtubules and actin-filaments were observed. The cells underwent apoptosis with an increase in caspase-3, and caspase-8 activities. The cell cycle was arrested at the G0/G1 phase under the treatment of C-PC/beta. In addition, the nuclear level of GAPDH decreased significantly. Decrease in the nuclear level of GAPDH prevents the cell cycle from entering into the S phase. Inhibition of cancer cell proliferation and induction of apoptosis may potentate the C-POP as a promising cancer prevention or therapy agent. (c) 2006 Elsevier Ireland Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Oncology |
| 类目[WOS] | Oncology |
| 研究领域[WOS] | Oncology |
| 关键词[WOS] | GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ; TUBULIN ; PROTEIN ; COMPONENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000244383900018 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8686]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Georgia State Univ, Georgia Canc Ctr, Dept Biol, Atlanta, GA 30303 USA 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Haizhen,Liu, Yongding,Gao, Xueliang,et al. The recombinant beta subunit of C-phycocyanin inhibits cell proliferation and induces apoptosis[J]. CANCER LETTERS,2007,247(1):150-158. |
| APA | Wang, Haizhen,Liu, Yongding,Gao, Xueliang,Carter, Christie L.,&Liu, Zhi-Ren.(2007).The recombinant beta subunit of C-phycocyanin inhibits cell proliferation and induces apoptosis.CANCER LETTERS,247(1),150-158. |
| MLA | Wang, Haizhen,et al."The recombinant beta subunit of C-phycocyanin inhibits cell proliferation and induces apoptosis".CANCER LETTERS 247.1(2007):150-158. |
入库方式： OAI收割
来源：水生生物研究所
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