Isolation and characterization of a rhabdovirus from co-infection of two viruses in mandarin fish
文献类型：期刊论文
| 作者 | Tao, Jian-Jun; Gui, Jian-Fang; Zhang, Qi-Ya |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2007-02-14 |
| 卷号 | 262期号:1页码:1-9 |
| 关键词 | mandarin fish co-infection rhabdovirus SCRV N gene Vesiculovirus |
| ISSN号 | 0044-8486 |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China |
| 中文摘要 | Co-infection of two viruses has been observed in mandarin fish (Siniperca chuatsi), but the two viruses have not been characterized. In this study, a rhabdovirus has been isolated from the co-infected two viruses extracted from the diseased mandarin fish, and its morphological structure and partial biochemical and biophysical characteristics have been observed and analyzed. The isolated rhabdovirus has a typical bullet shape, and is therefore called S. chttatsi rhabdovirus (SCRV). And, the isolated rhabdovirus produced a higher titer (10(8.5) TCID50 ml(-1)) than did the co-infecting viruses (10(6.5) TCID50 ml(-1)). Subsequently, the viral genome RNA was extracted, and used as template to clone the complete nucleoprotein (N) gene by RT-PCR amplification. Cloning and sequencing of the SCRV N protein revealed 42%-31% amino acid identities to that of trout rhabdovirus 903/87 and the rhabdoviruses in genus Vesiculovirus. SDS-PAGE separation of the isolated SCRV and other two rhabdoviruses also revealed obvious polypeptide profile difference. Moreover, the anti-SCRV N protein antibody was prepared, and the anti-SCRV N protein antibody only could recognize the SCRV N protein, whereas no antigenicity was detected in other two rhabdoviruses. The data suggested that the SCRV should be a rhabdovirus member related to the genus Vesiculovirus in the Rhabdoviridae. (c) 2006 Elsevier B.V. All rights reserved. |
| 英文摘要 | Co-infection of two viruses has been observed in mandarin fish (Siniperca chuatsi), but the two viruses have not been characterized. In this study, a rhabdovirus has been isolated from the co-infected two viruses extracted from the diseased mandarin fish, and its morphological structure and partial biochemical and biophysical characteristics have been observed and analyzed. The isolated rhabdovirus has a typical bullet shape, and is therefore called S. chttatsi rhabdovirus (SCRV). And, the isolated rhabdovirus produced a higher titer (10(8.5) TCID50 ml(-1)) than did the co-infecting viruses (10(6.5) TCID50 ml(-1)). Subsequently, the viral genome RNA was extracted, and used as template to clone the complete nucleoprotein (N) gene by RT-PCR amplification. Cloning and sequencing of the SCRV N protein revealed 42%-31% amino acid identities to that of trout rhabdovirus 903/87 and the rhabdoviruses in genus Vesiculovirus. SDS-PAGE separation of the isolated SCRV and other two rhabdoviruses also revealed obvious polypeptide profile difference. Moreover, the anti-SCRV N protein antibody was prepared, and the anti-SCRV N protein antibody only could recognize the SCRV N protein, whereas no antigenicity was detected in other two rhabdoviruses. The data suggested that the SCRV should be a rhabdovirus member related to the genus Vesiculovirus in the Rhabdoviridae. (c) 2006 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | HEMATOPOIETIC-NECROSIS-VIRUS ; VESICULAR STOMATITIS-VIRUS ; HEMORRHAGIC SEPTICEMIA VIRUS ; TROUT ONCORHYNCHUS-MYKISS ; N-GENE ; MOLECULAR CHARACTERIZATION ; NUCLEOTIDE-SEQUENCE ; GLYCOPROTEIN GENE ; SPRING VIREMIA ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000244384500001 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8688]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tao, Jian-Jun,Gui, Jian-Fang,Zhang, Qi-Ya. Isolation and characterization of a rhabdovirus from co-infection of two viruses in mandarin fish[J]. AQUACULTURE,2007,262(1):1-9. |
| APA | Tao, Jian-Jun,Gui, Jian-Fang,&Zhang, Qi-Ya.(2007).Isolation and characterization of a rhabdovirus from co-infection of two viruses in mandarin fish.AQUACULTURE,262(1),1-9. |
| MLA | Tao, Jian-Jun,et al."Isolation and characterization of a rhabdovirus from co-infection of two viruses in mandarin fish".AQUACULTURE 262.1(2007):1-9. |
入库方式： OAI收割
来源：水生生物研究所
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