Difterentially expressed genes of Tetrahymena thermophila in response to tributyltin (TBT) identified by suppression subtractive hybridization and real time quantitative PCR
文献类型：期刊论文
| 作者 | Feng, Lifang; Miao, Wei; Wu, Yuxuan |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2007-02-15 |
| 卷号 | 81期号:1页码:99-105 |
| ISSN号 | 0166-445X |
| 关键词 | Tetrahymena thermophila tributyltin suppression subtractive hybridization expressed sequence tags biomonitor |
| 通讯作者 | Miao, W, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Tributyltin (TBT) is widely used as antifouling paints, agriculture biocides, and plastic stabilizers around the world, resulting in great pollution problem in aquatic environments. However, it has been short of the biomonitor to detect TBT in freshwater. We constructed the suppression subtractive hybridization library of Tetrahymena thermophila exposed to TBT, and screened out 101 Expressed Sequence Tags whose expressions were significantly up- or down-regulated with TBT treatment. From this, a series of genes related to the TBT toxicity were discovered, such as glutathione-S-transferase gene (down-regulated), plasma membrane Ca2+ ATPase isoforms 3 gene (up-regulated) and NgoA (up-regulated). Furthermore, their expressions under different concentrations of TBT treatment (0.5-40 ppb) were detected by real time fluorescent quantitative PCR. The differentially expressed genes of T thermophila in response to TBT were identified, which provide the basic to make Tetrahymena as a sensitive, rapid and convenient TBT biomonitor in freshwater based on rDNA inducible expression system. (c) 2006 Elsevier B.V. All rights reserved. |
| 英文摘要 | Tributyltin (TBT) is widely used as antifouling paints, agriculture biocides, and plastic stabilizers around the world, resulting in great pollution problem in aquatic environments. However, it has been short of the biomonitor to detect TBT in freshwater. We constructed the suppression subtractive hybridization library of Tetrahymena thermophila exposed to TBT, and screened out 101 Expressed Sequence Tags whose expressions were significantly up- or down-regulated with TBT treatment. From this, a series of genes related to the TBT toxicity were discovered, such as glutathione-S-transferase gene (down-regulated), plasma membrane Ca2+ ATPase isoforms 3 gene (up-regulated) and NgoA (up-regulated). Furthermore, their expressions under different concentrations of TBT treatment (0.5-40 ppb) were detected by real time fluorescent quantitative PCR. The differentially expressed genes of T thermophila in response to TBT were identified, which provide the basic to make Tetrahymena as a sensitive, rapid and convenient TBT biomonitor in freshwater based on rDNA inducible expression system. (c) 2006 Elsevier B.V. All rights reserved. |
| 学科主题 | Marine & Freshwater Biology; Toxicology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | ORGANOTIN COMPOUNDS ; DREISSENA-POLYMORPHA ; POLLUTION ; BUTYLTIN ; EMBRYOS ; TRANSFERASES ; ENVIRONMENT ; L. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000244173200011 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8702]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Wuhan Branch, Wuhan 430072, Peoples R China 3.Wuhan Univ, Sch Life Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Lifang,Miao, Wei,Wu, Yuxuan. Difterentially expressed genes of Tetrahymena thermophila in response to tributyltin (TBT) identified by suppression subtractive hybridization and real time quantitative PCR[J]. AQUATIC TOXICOLOGY,2007,81(1):99-105. |
| APA | Feng, Lifang,Miao, Wei,&Wu, Yuxuan.(2007).Difterentially expressed genes of Tetrahymena thermophila in response to tributyltin (TBT) identified by suppression subtractive hybridization and real time quantitative PCR.AQUATIC TOXICOLOGY,81(1),99-105. |
| MLA | Feng, Lifang,et al."Difterentially expressed genes of Tetrahymena thermophila in response to tributyltin (TBT) identified by suppression subtractive hybridization and real time quantitative PCR".AQUATIC TOXICOLOGY 81.1(2007):99-105. |
入库方式： OAI收割
来源：水生生物研究所
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