热门
Differential and spermatogenic cell-specific expression of DMRT1 during sex reversal in protogynous hermaphroditic groupers
文献类型：期刊论文
| 作者 | Xia, Wei; Zhou, Li; Yao, Bo; Li, Chuang-Ju; Gui, Han-Fang |
| 刊名 | MOLECULAR AND CELLULAR ENDOCRINOLOGY
 |
| 出版日期 | 2007-01-15 |
| 卷号 | 263期号:1-2页码:156-172 |
| 关键词 | DMRT1 Sox3 Sox9 grouper gonad sex differentiation sex reversal spermatogenesis |
| ISSN号 | 0303-7207 |
| 通讯作者 | Gui, HF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | DMRT1 has been suggested to play different roles in sex determination and gonad differentiation, because different expression patterns have been reported among different vertebrates. The groupers, since their gonads first develop as ovary and then reverse into testis, have been thought as good models to study sex differentiation and determination. In this study, we cloned the full-length cDNAs of DMRT] gene from orange-spotted grouper (Epinephelus coioides), and prepared corresponding anti-EcDMRT1] antiserum to study the relationship of DMRT] to sex reversal. One important finding is that the grouper DMRT] is not only differentially expressed in different stage gonads, but also restricted to specific stages and specific cells of spermatogenesis. Grouper DMRT1 protein exists only in spermatogonia, primary spermatocytes and secondary spermatocytes, but not in the supporting Sertoli cells. Moreover, we confirmed that EcSox3 is expressed not only in oogonia and different stage oocytes, but also in Sertoli cells and spermatogonia, and EcSox9 is expressed only in Sertoli cells. The data suggested that grouper DMRT1 might be a more specific sex differentiation gene for spermatogenesis, and play its role at the specific stages from spermatogonia to spermatocytes. In addition, no introns were found in the grouper DMRT1, and no duplicated DMRT1, genes were detected. The finding implicates that the intronless DMRT1 that is able to undergo rapid transcriptional turnover might be a significant gene for stimulating spermatogenesis in the protogynous hermaphroditic gonad. (c) 2006 Published by Elsevier Ireland Ltd. |
| 英文摘要 | DMRT1 has been suggested to play different roles in sex determination and gonad differentiation, because different expression patterns have been reported among different vertebrates. The groupers, since their gonads first develop as ovary and then reverse into testis, have been thought as good models to study sex differentiation and determination. In this study, we cloned the full-length cDNAs of DMRT] gene from orange-spotted grouper (Epinephelus coioides), and prepared corresponding anti-EcDMRT1] antiserum to study the relationship of DMRT] to sex reversal. One important finding is that the grouper DMRT] is not only differentially expressed in different stage gonads, but also restricted to specific stages and specific cells of spermatogenesis. Grouper DMRT1 protein exists only in spermatogonia, primary spermatocytes and secondary spermatocytes, but not in the supporting Sertoli cells. Moreover, we confirmed that EcSox3 is expressed not only in oogonia and different stage oocytes, but also in Sertoli cells and spermatogonia, and EcSox9 is expressed only in Sertoli cells. The data suggested that grouper DMRT1 might be a more specific sex differentiation gene for spermatogenesis, and play its role at the specific stages from spermatogonia to spermatocytes. In addition, no introns were found in the grouper DMRT1, and no duplicated DMRT1, genes were detected. The finding implicates that the intronless DMRT1 that is able to undergo rapid transcriptional turnover might be a significant gene for stimulating spermatogenesis in the protogynous hermaphroditic gonad. (c) 2006 Published by Elsevier Ireland Ltd. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Cell Biology; Endocrinology & Metabolism |
| 类目[WOS] | Cell Biology ; Endocrinology & Metabolism |
| 研究领域[WOS] | Cell Biology ; Endocrinology & Metabolism |
| 关键词[WOS] | PROTANDROUS BLACK PORGY ; ANTI-MULLERIAN HORMONE ; GONADAL DIFFERENTIATION ; MOLECULAR-CLONING ; ORYZIAS-LATIPES ; ACANTHOPAGRUS-SCHLEGELI ; GENE-EXPRESSION ; CHICKEN EMBRYOS ; MEDAKA FISH ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243476800017 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8726]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Wei,Zhou, Li,Yao, Bo,et al. Differential and spermatogenic cell-specific expression of DMRT1 during sex reversal in protogynous hermaphroditic groupers[J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY,2007,263(1-2):156-172. |
| APA | Xia, Wei,Zhou, Li,Yao, Bo,Li, Chuang-Ju,&Gui, Han-Fang.(2007).Differential and spermatogenic cell-specific expression of DMRT1 during sex reversal in protogynous hermaphroditic groupers.MOLECULAR AND CELLULAR ENDOCRINOLOGY,263(1-2),156-172. |
| MLA | Xia, Wei,et al."Differential and spermatogenic cell-specific expression of DMRT1 during sex reversal in protogynous hermaphroditic groupers".MOLECULAR AND CELLULAR ENDOCRINOLOGY 263.1-2(2007):156-172. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。