Cloning and expression of medaka dazl during ernbryogenesis and gametogenesis
文献类型：期刊论文
| 作者 | Xu, Hongyan; Li, Mingyou; Gui, Jianfang; Hong, Yunhan |
| 刊名 | GENE EXPRESSION PATTERNS
 |
| 出版日期 | 2007 |
| 卷号 | 7期号:3页码:332-338 |
| ISSN号 | 1567-133X |
| 关键词 | dazl germ cells medaka meiosis RNA-binding protein spermatogenesis |
| 通讯作者 | Hong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore |
| 中文摘要 | The Deleted in azoospermia family consists of RNA-binding proteins Bottle, Daz, and Daz-like (Dazl) that are expressed in the germline. Here, we report the cloning and expression of the medakafish (Oryzias latipes) dazl gene (odazl). Interestingly, although the predicted medaka Dazl protein (oDazl) contains a RRM motif and a DAZ repeat characteristic of its mammalian homologs, it lacks 80 aa at the C-terminus. By RT-PCR, RNA in situ hybridization, Western blotting and fluorescent immunohistochemistry using a rabbit anti-DazI antibody (alpha Oazl), we analyzed the expression patterns of odazl and its protein. The odazl transcript persists throughout embryogenesis and delineates with primordial germ cells. In adults, the expression of odazl RNA and its protein is restricted to germ cells of both the testis and ovary. We observed differential expression of RNA and protein at critical stages of gametogenesis. In the testis, the odazl RNA is low at premeiotic stages, abundant at meiotic stages, but absent in postmeiotic stages; whereas the oDazl protein is rich in premeiotic stages, reduced at meiotic stages, becomes barely detectable or absent in postmeiotic round spermatids or sperm, respectively. This is in sharp mature spermatozoa. In the ovary, the odazl RNA contrast to the human situation where the Dazl transcript and protein are present in and protein persist throughout oogenesis and also show differential expression at premeiotic, meiotic and postmeiotic stages. Thus, the odazl or its protein is a marker for germ cells during embryogenesis and at critical stages of gametogenesis in both sexes of medaka. (c) 2006 Elsevier B.V. All rights reserved. |
| 英文摘要 | The Deleted in azoospermia family consists of RNA-binding proteins Bottle, Daz, and Daz-like (Dazl) that are expressed in the germline. Here, we report the cloning and expression of the medakafish (Oryzias latipes) dazl gene (odazl). Interestingly, although the predicted medaka Dazl protein (oDazl) contains a RRM motif and a DAZ repeat characteristic of its mammalian homologs, it lacks 80 aa at the C-terminus. By RT-PCR, RNA in situ hybridization, Western blotting and fluorescent immunohistochemistry using a rabbit anti-DazI antibody (alpha Oazl), we analyzed the expression patterns of odazl and its protein. The odazl transcript persists throughout embryogenesis and delineates with primordial germ cells. In adults, the expression of odazl RNA and its protein is restricted to germ cells of both the testis and ovary. We observed differential expression of RNA and protein at critical stages of gametogenesis. In the testis, the odazl RNA is low at premeiotic stages, abundant at meiotic stages, but absent in postmeiotic stages; whereas the oDazl protein is rich in premeiotic stages, reduced at meiotic stages, becomes barely detectable or absent in postmeiotic round spermatids or sperm, respectively. This is in sharp mature spermatozoa. In the ovary, the odazl RNA contrast to the human situation where the Dazl transcript and protein are present in and protein persist throughout oogenesis and also show differential expression at premeiotic, meiotic and postmeiotic stages. Thus, the odazl or its protein is a marker for germ cells during embryogenesis and at critical stages of gametogenesis in both sexes of medaka. (c) 2006 Elsevier B.V. All rights reserved. |
| 学科主题 | Developmental Biology; Genetics & Heredity |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Developmental Biology ; Genetics & Heredity |
| 研究领域[WOS] | Developmental Biology ; Genetics & Heredity |
| 关键词[WOS] | PRIMORDIAL GERM-CELLS ; ORYZIAS-LATIPES ; GENE ENCODES ; HOMOLOG ; RNA ; PROTEIN ; PLASM ; SPERMATOGENESIS ; DIFFERENTIATION ; XENOPUS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243540300017 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8728]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Hongyan,Li, Mingyou,Gui, Jianfang,et al. Cloning and expression of medaka dazl during ernbryogenesis and gametogenesis[J]. GENE EXPRESSION PATTERNS,2007,7(3):332-338. |
| APA | Xu, Hongyan,Li, Mingyou,Gui, Jianfang,&Hong, Yunhan.(2007).Cloning and expression of medaka dazl during ernbryogenesis and gametogenesis.GENE EXPRESSION PATTERNS,7(3),332-338. |
| MLA | Xu, Hongyan,et al."Cloning and expression of medaka dazl during ernbryogenesis and gametogenesis".GENE EXPRESSION PATTERNS 7.3(2007):332-338. |
入库方式： OAI收割
来源：水生生物研究所
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