Characterization and application of monoclonal antibodies against turbot (Scophthalmus maximus) rhabdovirus
文献类型：期刊论文
| 作者 | Zhou, Guang-Zhou; Li, Zheng-Qiu; Zhang, Qi-Ya |
| 刊名 | VIRAL IMMUNOLOGY
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 19期号:4页码:637-645 |
| ISSN号 | 0882-8245 |
| 关键词 | HEMATOPOIETIC NECROSIS VIRUS HEMORRHAGIC SEPTICEMIA VIRUS COMPLETE GENOMIC SEQUENCE SCALLOP CHLAMYS-FARRERI FLOW-CYTOMETRY PARALICHTHYS-OLIVACEUS FISH RHABDOVIRUS SPRING VIREMIA DISEASE-VIRUS MARINE FISH |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Five monoclonal antibodies (mAbs), 1G8, 1H9, 2D2, 2D3, and 2F5, against Scophthalmus maximus rhabdovirus (SMRV) were prepared. Characterization of the mAbs included indirect enzyme-linked immunosorbent assay, isotyping, viral inhibition assay, immunofluorescence staining of virus-infected cell cultures, and Western blot analysis. Isotyping revealed that 1G8 and 1H9 were of the IgG2b subclass and that the other three were IgM. 2D2, 2D3, and 2F5 partially inhibited SMRV infection in epithelioma. papulosum cyprinid (EPC) cell culture. Western blotting showed that all five mAbs could react with two SMRV proteins with molecular masses of approximately 30 kDa (P) and 26 kDa (M). These two proteins were localized within the cytoplasm of SMRV-infected EPC cells by immunofluorescence assay. Also, progressive foci of viral replication in cell cultures were monitored from 6 to 24 h, using mAb 2D3 as the primary antibody. A flow cytometry procedure was used to detect and quantify SMRV-infected (0.01 PFU/cell) EPC cells with mAb 2D3, and 10.8% of cells could be distinguished as infected 36 h postinfection. Moreover, mAb 2D3 was successfully applied for the detection of viral antigen in cryosections from flounder tissues by immunohistochemistry tests. |
| 英文摘要 | Five monoclonal antibodies (mAbs), 1G8, 1H9, 2D2, 2D3, and 2F5, against Scophthalmus maximus rhabdovirus (SMRV) were prepared. Characterization of the mAbs included indirect enzyme-linked immunosorbent assay, isotyping, viral inhibition assay, immunofluorescence staining of virus-infected cell cultures, and Western blot analysis. Isotyping revealed that 1G8 and 1H9 were of the IgG2b subclass and that the other three were IgM. 2D2, 2D3, and 2F5 partially inhibited SMRV infection in epithelioma. papulosum cyprinid (EPC) cell culture. Western blotting showed that all five mAbs could react with two SMRV proteins with molecular masses of approximately 30 kDa (P) and 26 kDa (M). These two proteins were localized within the cytoplasm of SMRV-infected EPC cells by immunofluorescence assay. Also, progressive foci of viral replication in cell cultures were monitored from 6 to 24 h, using mAb 2D3 as the primary antibody. A flow cytometry procedure was used to detect and quantify SMRV-infected (0.01 PFU/cell) EPC cells with mAb 2D3, and 10.8% of cells could be distinguished as infected 36 h postinfection. Moreover, mAb 2D3 was successfully applied for the detection of viral antigen in cryosections from flounder tissues by immunohistochemistry tests. |
| 学科主题 | Immunology; Virology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Virology |
| 研究领域[WOS] | Immunology ; Virology |
| 关键词[WOS] | HEMATOPOIETIC NECROSIS VIRUS ; HEMORRHAGIC SEPTICEMIA VIRUS ; COMPLETE GENOMIC SEQUENCE ; SCALLOP CHLAMYS-FARRERI ; FLOW-CYTOMETRY ; PARALICHTHYS-OLIVACEUS ; FISH RHABDOVIRUS ; SPRING VIREMIA ; DISEASE-VIRUS ; MARINE FISH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243346600006 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8730]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Guang-Zhou,Li, Zheng-Qiu,Zhang, Qi-Ya. Characterization and application of monoclonal antibodies against turbot (Scophthalmus maximus) rhabdovirus[J]. VIRAL IMMUNOLOGY,2006,19(4):637-645. |
| APA | Zhou, Guang-Zhou,Li, Zheng-Qiu,&Zhang, Qi-Ya.(2006).Characterization and application of monoclonal antibodies against turbot (Scophthalmus maximus) rhabdovirus.VIRAL IMMUNOLOGY,19(4),637-645. |
| MLA | Zhou, Guang-Zhou,et al."Characterization and application of monoclonal antibodies against turbot (Scophthalmus maximus) rhabdovirus".VIRAL IMMUNOLOGY 19.4(2006):637-645. |
入库方式： OAI收割
来源：水生生物研究所
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