Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening
文献类型：期刊论文
| 作者 | Yuan, Li; Zhang, Xiaohua; Xiao, Nan; Dai, Lingfen; Chen, Weizhou; Jia, Chensong; Zhao, Ruohong; Hemmingsen, Sean M.; Dai, Heping |
| 刊名 | DISEASES OF AQUATIC ORGANISMS
 |
| 出版日期 | 2006-10-17 |
| 卷号 | 72期号:2页码:93-99 |
| 关键词 | white spot syndrome virus (WSSV) phage display neutralizing antibody single chain fragment variable regions (scFv) antibody |
| ISSN号 | 0177-5103 |
| 通讯作者 | Dai, HP, Chinese Acad Sci, Inst Hydrobiol, 7 E Lake So Rd, Wuhan 430072, Peoples R China |
| 中文摘要 | White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease. |
| 英文摘要 | White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp during the last decade, we still lack methods either to prevent or cure white spot disease. Most of the studies on neutralizing antibodies from sera have been performed using in vivo assays. For the first time, we report use of an in vitro screening method to obtain a neutralizing scFv antibody against WSSV from a previously constructed anti-WSSV single chain fragment variable region (scFv) antibody phage display library. From clones that were positive for WSSV by ELISA, 1 neutralizing scFv antibody was identified using an in vitro screening method based on shrimp primary lymphoid cell cultures. The availability of a neutralizing antibody against the virus should accelerate identification of infection-related genes and the host cell receptor, and may also enable new approaches to the prevention and cure of white spot disease. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Veterinary Sciences |
| 关键词[WOS] | WHITE-SPOT-SYNDROME ; SYNDROME-VIRUS WSSV ; PRAWN PENAEUS-MONODON ; MONOCLONAL-ANTIBODIES ; CAMBARUS-CLARKII ; GENOME SEQUENCE ; CELL-CULTURES ; SHRIMP ; PROTEIN ; INFECTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000242930700001 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8742]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Shantou Univ, Key Lab Marine Biol Guangdong Province, Marine Biol Lab Nanao Stn, Shantou 515900, Peoples R China 4.Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada |
| 推荐引用方式 GB/T 7714 | Yuan, Li,Zhang, Xiaohua,Xiao, Nan,et al. Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening[J]. DISEASES OF AQUATIC ORGANISMS,2006,72(2):93-99. |
| APA | Yuan, Li.,Zhang, Xiaohua.,Xiao, Nan.,Dai, Lingfen.,Chen, Weizhou.,...&Dai, Heping.(2006).Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening.DISEASES OF AQUATIC ORGANISMS,72(2),93-99. |
| MLA | Yuan, Li,et al."Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening".DISEASES OF AQUATIC ORGANISMS 72.2(2006):93-99. |
入库方式： OAI收割
来源：水生生物研究所
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