The acute effects of microcystin LR on the transcription of nine glutathione S-transferase genes in common carp Cyprinus carpio L.
文献类型：期刊论文
| 作者 | Fu, Juan; Xie, Ping |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2006-12-01 |
| 卷号 | 80期号:3页码:261-266 |
| ISSN号 | 0166-445X |
| 关键词 | microcystin glutathione S-transferases real time PCR common carp Cyprinus carpio |
| 通讯作者 | Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China |
| 中文摘要 | The glutathione S-transferases play important roles in the detoxification of microcystin. In this experiment, nine glutathione S-transferase genes including cytosolic GSTs (rho, mu, theta, alpha and pi), mitochondrial GST (kappa) and microsomal GSTs (mGST1, mGST2 and mGST3) were cloned from common carp Cyprinus carpio. The mRNA abundance of each carp GST isoform in liver was analyzed by real time PCR. The relative changes after stimulation with microcystin LR were also analyzed: increased levels of transcription of GST alpha, rho and mGST3 isoforms were detected at 6 h post stimulation; the transcription of mu, theta and mGST2 isoforms were relatively stable; and all the GST isoforms except GST kappa and rho recovered to original levels compared with controls at 72 h. It is suggested that MC-LR showed different effects on the transcription of nine carp GST isoforms. (c) 2006 Elsevier B.V. All rights reserved. |
| 英文摘要 | The glutathione S-transferases play important roles in the detoxification of microcystin. In this experiment, nine glutathione S-transferase genes including cytosolic GSTs (rho, mu, theta, alpha and pi), mitochondrial GST (kappa) and microsomal GSTs (mGST1, mGST2 and mGST3) were cloned from common carp Cyprinus carpio. The mRNA abundance of each carp GST isoform in liver was analyzed by real time PCR. The relative changes after stimulation with microcystin LR were also analyzed: increased levels of transcription of GST alpha, rho and mGST3 isoforms were detected at 6 h post stimulation; the transcription of mu, theta and mGST2 isoforms were relatively stable; and all the GST isoforms except GST kappa and rho recovered to original levels compared with controls at 72 h. It is suggested that MC-LR showed different effects on the transcription of nine carp GST isoforms. (c) 2006 Elsevier B.V. All rights reserved. |
| 学科主题 | Marine & Freshwater Biology; Toxicology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | PLAICE PLEURONECTES-PLATESSA ; PROTEIN PHOSPHATASES ; RAINBOW-TROUT ; DETOXIFICATION ; EXPRESSION ; CONJUGATE ; LIVER ; FISH ; IDENTIFICATION ; DETOXICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000242776900006 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8748]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Juan,Xie, Ping. The acute effects of microcystin LR on the transcription of nine glutathione S-transferase genes in common carp Cyprinus carpio L.[J]. AQUATIC TOXICOLOGY,2006,80(3):261-266. |
| APA | Fu, Juan,&Xie, Ping.(2006).The acute effects of microcystin LR on the transcription of nine glutathione S-transferase genes in common carp Cyprinus carpio L..AQUATIC TOXICOLOGY,80(3),261-266. |
| MLA | Fu, Juan,et al."The acute effects of microcystin LR on the transcription of nine glutathione S-transferase genes in common carp Cyprinus carpio L.".AQUATIC TOXICOLOGY 80.3(2006):261-266. |
入库方式： OAI收割
来源：水生生物研究所
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