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Identification and characterization of a cathepsin L-like cysteine protease from Taenia solium metacestode
文献类型：期刊论文
| 作者 | Li, Ai Hua; Moon, Sung-Ung; Park, Yun-Kyu; Na, Byoung-Kuk; Hwang, Myung-Gi; Oh, Chang-Mi; Cho, Shin-Hyeong; Kong, Yoon; Kim, Tong-Soo; Chung, Pyung-Rim |
| 刊名 | VETERINARY PARASITOLOGY
 |
| 出版日期 | 2006-11-05 |
| 卷号 | 141期号:3-4页码:251-259 |
| 关键词 | Taenia solium metacestode cysteine protease recombinant protein |
| ISSN号 | 0304-4017 |
| 通讯作者 | Chung, PR, Inha Univ, Coll Med, Dept Parasitol, Inchon 400712, South Korea |
| 中文摘要 | Taenia solium metacestode, a larval pork tapeworm, is a causative agent of neurocysticercosis, one of the most common parasitic diseases in the human central nervous system. In this study, we identified a cDNA encoding for a cathepsin L-like cysteine protease from the T solium metacestode (TsCL-1) and characterized the biochemical properties of the recombinant enzyme. The cloned cDNA of 1216 bp encoded 339 amino acids with an approximate molecular weight of 37.6 kDa which containing a typical signal peptide sequence (17 amino acids), a pro-domain (106 amino acids), and a mature domain (216 amino acids). Sequence alignments of TsCL-1 showed low sequence similarity of 27.3-44.6 to cathepsin L-like cysteine proteases from other helminth parasites, but the similarity was increased to 35.9-55.0 when compared to mature domains. The bacterially expressed recombinant protein (rTsCL-1) did not show enzyme activity; however, the rTsCL-1 expressed in Pichia pastoris showed typical biochemical characteristics of cysteine proteases. It degraded human immunoglobulin G (IgG) and bovine serum albumin (BSA), but not collagen. Western blot analysis of the rTsCL-1 showed antigenicity against the sera from patients with cysticercosis, sparganosis or fascioliasis, but weak or no antigenicity against the sera from patients with paragonimiasis or clonorchiasis. (c) 2006 Published by Elsevier B.V. |
| 英文摘要 | Taenia solium metacestode, a larval pork tapeworm, is a causative agent of neurocysticercosis, one of the most common parasitic diseases in the human central nervous system. In this study, we identified a cDNA encoding for a cathepsin L-like cysteine protease from the T solium metacestode (TsCL-1) and characterized the biochemical properties of the recombinant enzyme. The cloned cDNA of 1216 bp encoded 339 amino acids with an approximate molecular weight of 37.6 kDa which containing a typical signal peptide sequence (17 amino acids), a pro-domain (106 amino acids), and a mature domain (216 amino acids). Sequence alignments of TsCL-1 showed low sequence similarity of 27.3-44.6 to cathepsin L-like cysteine proteases from other helminth parasites, but the similarity was increased to 35.9-55.0 when compared to mature domains. The bacterially expressed recombinant protein (rTsCL-1) did not show enzyme activity; however, the rTsCL-1 expressed in Pichia pastoris showed typical biochemical characteristics of cysteine proteases. It degraded human immunoglobulin G (IgG) and bovine serum albumin (BSA), but not collagen. Western blot analysis of the rTsCL-1 showed antigenicity against the sera from patients with cysticercosis, sparganosis or fascioliasis, but weak or no antigenicity against the sera from patients with paragonimiasis or clonorchiasis. (c) 2006 Published by Elsevier B.V. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Parasitology; Veterinary Sciences |
| 类目[WOS] | Parasitology ; Veterinary Sciences |
| 研究领域[WOS] | Parasitology ; Veterinary Sciences |
| 关键词[WOS] | PARAGONIMUS-WESTERMANI ; PROTEINASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000241720000007 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8802]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Inha Univ, Coll Med, Dept Parasitol, Inchon 400712, South Korea 2.Korea Ctr Dis Control & Prevent, Natl Inst Hlth, Dib Malaria & Parasit Dis, Seoul 122701, South Korea 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 4.Gyeongsang Natl Univ, Coll Med, Dept Parasitol, Jinju 660751, South Korea 5.Gyeongsang Natl Univ, Coll Med, Inst Hlth Sci, Jinju 660751, South Korea 6.Sungkyunkwan Univ, Sch Med, Suwon 440746, South Korea 7.Samsung Biomed Res Inst, Dept Mol Parasitol, Suwon 440746, South Korea 8.Samsung Biomed Res Inst, Ctr Mol Med, Suwon 440746, South Korea |
| 推荐引用方式 GB/T 7714 | Li, Ai Hua,Moon, Sung-Ung,Park, Yun-Kyu,et al. Identification and characterization of a cathepsin L-like cysteine protease from Taenia solium metacestode[J]. VETERINARY PARASITOLOGY,2006,141(3-4):251-259. |
| APA | Li, Ai Hua.,Moon, Sung-Ung.,Park, Yun-Kyu.,Na, Byoung-Kuk.,Hwang, Myung-Gi.,...&Chung, Pyung-Rim.(2006).Identification and characterization of a cathepsin L-like cysteine protease from Taenia solium metacestode.VETERINARY PARASITOLOGY,141(3-4),251-259. |
| MLA | Li, Ai Hua,et al."Identification and characterization of a cathepsin L-like cysteine protease from Taenia solium metacestode".VETERINARY PARASITOLOGY 141.3-4(2006):251-259. |
入库方式： OAI收割
来源：水生生物研究所
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