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Analysis of steric mass-action model for protein adsorption equilibrium onto porous anion-exchange adsorbent
文献类型：期刊论文
| 作者 | Chen, Wei-Dong; Hu, Han-Hua; Wang, Yan-Dong |
| 刊名 | CHEMICAL ENGINEERING SCIENCE
 |
| 出版日期 | 2006-11-01 |
| 卷号 | 61期号:21页码:7068-7076 |
| ISSN号 | 0009-2509 |
| 关键词 | ion exchange adsorption isothermal protein simulation separations |
| 通讯作者 | Chen, WD, China Univ Petr Beijing, Fac Chem Sci & Engn, Dept Biochem Engn, Beijing 102249, Peoples R China |
| 中文摘要 | The ion-exchange equilibrium of bovine serum albumin (BSA) to an anion exchanger, DEAE Spherodex M, has been studied by batch adsorption experiments at pH values ranging from 5.26 to 7.6 and ionic strengths from 10 to 117.1 mmol/l. Using the unadjustable adsorption equilibrium parameters obtained from batch experiments, the applicability of the steric mass-action (SMA) model was analyzed for describing protein ion-exchange equilibrium in different buffer systems. The parametric sensitivity analysis was performed by perturbing each of the model parameters, while holding the rest constant. The simulation results showed that, at high salt concentrations or low pHs close to the isoelectric point of the protein, the precision of the model prediction decreased. Parametric sensitivity analysis showed that the characteristic charge and protein steric factor had the largest effects on ion-exchange equilibrium, while the effect of equilibrium constant was about 70%-95% smaller than those of characteristic charge and steric factor under all conditions investigated. The SMA model with the relationship between the adjusted characteristic charge and the salt concentration can well predict the protein adsorption isotherms in a wide pH range from 5.84 to 7.6. It is considered that the SMA model could be further improved by taking into account the effect of salt concentration on the intermolecular interactions of proteins. (c) 2006 Elsevier Ltd. All rights reserved. |
| 英文摘要 | The ion-exchange equilibrium of bovine serum albumin (BSA) to an anion exchanger, DEAE Spherodex M, has been studied by batch adsorption experiments at pH values ranging from 5.26 to 7.6 and ionic strengths from 10 to 117.1 mmol/l. Using the unadjustable adsorption equilibrium parameters obtained from batch experiments, the applicability of the steric mass-action (SMA) model was analyzed for describing protein ion-exchange equilibrium in different buffer systems. The parametric sensitivity analysis was performed by perturbing each of the model parameters, while holding the rest constant. The simulation results showed that, at high salt concentrations or low pHs close to the isoelectric point of the protein, the precision of the model prediction decreased. Parametric sensitivity analysis showed that the characteristic charge and protein steric factor had the largest effects on ion-exchange equilibrium, while the effect of equilibrium constant was about 70%-95% smaller than those of characteristic charge and steric factor under all conditions investigated. The SMA model with the relationship between the adjusted characteristic charge and the salt concentration can well predict the protein adsorption isotherms in a wide pH range from 5.84 to 7.6. It is considered that the SMA model could be further improved by taking into account the effect of salt concentration on the intermolecular interactions of proteins. (c) 2006 Elsevier Ltd. All rights reserved. |
| 学科主题 | Engineering ; Chemical |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Chemical |
| 研究领域[WOS] | Engineering |
| 关键词[WOS] | BOVINE SERUM-ALBUMIN ; ION-EXCHANGE ; AQUEOUS-SOLUTIONS ; CHROMATOGRAPHY ; RETENTION ; DIFFUSION ; KINETICS ; OPTIMIZATION ; SEPARATIONS ; SIMULATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000241446700017 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8814]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.China Univ Petr Beijing, Fac Chem Sci & Engn, Dept Biochem Engn, Beijing 102249, Peoples R China 2.Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, Tianjin 300072, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Wei-Dong,Hu, Han-Hua,Wang, Yan-Dong. Analysis of steric mass-action model for protein adsorption equilibrium onto porous anion-exchange adsorbent[J]. CHEMICAL ENGINEERING SCIENCE,2006,61(21):7068-7076. |
| APA | Chen, Wei-Dong,Hu, Han-Hua,&Wang, Yan-Dong.(2006).Analysis of steric mass-action model for protein adsorption equilibrium onto porous anion-exchange adsorbent.CHEMICAL ENGINEERING SCIENCE,61(21),7068-7076. |
| MLA | Chen, Wei-Dong,et al."Analysis of steric mass-action model for protein adsorption equilibrium onto porous anion-exchange adsorbent".CHEMICAL ENGINEERING SCIENCE 61.21(2006):7068-7076. |
入库方式： OAI收割
来源：水生生物研究所
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