Improvement in growth and toxin production of Alexandrium tamarense by two-step culture method
文献类型：期刊论文
| 作者 | Hu, Hanhua; Shi, Yanjun; Cong, Wei |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 18期号:2页码:119-126 |
| 关键词 | airlift photobioreactor Alexandrium tamarense growth saxitoxin two-step culture |
| ISSN号 | 0921-8971 |
| 通讯作者 | Hu, HH, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China |
| 中文摘要 | The growth and toxin content of the dinoflagellate Alexandrium tamarense ATHK was markedly affected by culture methods. In early growth phase at lower cell density static or mild agitation methods were beneficial to growth, but continuous agitation or aeration, to some extent, had an adverse effect on cell growth. Static culture in 2 L Erlenmeyer flasks had the highest growth rate (0.38 d(-1)) but smaller cell size compared with other culture conditions. Cells grown under aerated conditions possessed low nitrogen and phosphorus cell yields, namely high N and P cell-quota. At day 18, cells grown in continuous agitated and 1 h aerated culture entered the late stationary phase and their cellular toxin contents were higher (0.67 and 0.54 pg cell(-1)) compared with cells grown by other culture methods (0.27-0.49 pg cell(-1)). The highest cell density and cellular toxin content were 17190 cells mL(-1) and 1.26 pg cell(-1) respectively in an airlift photobioreactor with two-step culture. The results indicate that A. tamarense could be grown successfully in airlift photobioreactor by a two-step culture method, which involved cultivating the cells statically for 4 days and then aerating the medium. This provides an efficient way to enhance cell and toxin yield of A. tamarense. |
| 英文摘要 | The growth and toxin content of the dinoflagellate Alexandrium tamarense ATHK was markedly affected by culture methods. In early growth phase at lower cell density static or mild agitation methods were beneficial to growth, but continuous agitation or aeration, to some extent, had an adverse effect on cell growth. Static culture in 2 L Erlenmeyer flasks had the highest growth rate (0.38 d(-1)) but smaller cell size compared with other culture conditions. Cells grown under aerated conditions possessed low nitrogen and phosphorus cell yields, namely high N and P cell-quota. At day 18, cells grown in continuous agitated and 1 h aerated culture entered the late stationary phase and their cellular toxin contents were higher (0.67 and 0.54 pg cell(-1)) compared with cells grown by other culture methods (0.27-0.49 pg cell(-1)). The highest cell density and cellular toxin content were 17190 cells mL(-1) and 1.26 pg cell(-1) respectively in an airlift photobioreactor with two-step culture. The results indicate that A. tamarense could be grown successfully in airlift photobioreactor by a two-step culture method, which involved cultivating the cells statically for 4 days and then aerating the medium. This provides an efficient way to enhance cell and toxin yield of A. tamarense. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biotechnology & Applied Microbiology; Marine & Freshwater Biology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 关键词[WOS] | SMALL-SCALE TURBULENCE ; PHOSPHORUS LIMITATION ; NATURAL PHYTOPLANKTON ; C2 TOXIN ; DINOFLAGELLATE ; DINOPHYCEAE ; TOXICITY ; MINUTUM ; NITRATE ; INCREASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239891700002 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8872]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China 2.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Hanhua,Shi, Yanjun,Cong, Wei. Improvement in growth and toxin production of Alexandrium tamarense by two-step culture method[J]. JOURNAL OF APPLIED PHYCOLOGY,2006,18(2):119-126. |
| APA | Hu, Hanhua,Shi, Yanjun,&Cong, Wei.(2006).Improvement in growth and toxin production of Alexandrium tamarense by two-step culture method.JOURNAL OF APPLIED PHYCOLOGY,18(2),119-126. |
| MLA | Hu, Hanhua,et al."Improvement in growth and toxin production of Alexandrium tamarense by two-step culture method".JOURNAL OF APPLIED PHYCOLOGY 18.2(2006):119-126. |
入库方式： OAI收割
来源：水生生物研究所
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