Upstream regulatory region of zebrafish lunatic fringe: Isolation and promoter analysis
文献类型：期刊论文
| 作者 | Liu, Jing; Sun, Yong-Hua; Wang, Na; Wang, Ya-Ping; Zhu, Zuo-Yan |
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2006-07-01 |
| 卷号 | 8期号:4页码:357-365 |
| 关键词 | green fluorescent protein lfng maternal factor promoter zebrafish |
| ISSN号 | 1436-2228 |
| 通讯作者 | Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Lunatic fringe (Lfng), one modulator of Notch signaling, plays an essential part in demarcation of tissues boundaries during animal early development, especially somitogenesis. To characterize the promoter of zebrafish 1fng and generate somite-specific transgenic zebrafish, we isolated the upstream regulatory region of zebrafish 1fng by blast search at the Ensembl genome database (http://www. ensembl.org) and analyzed the promoter activity using green fluorescent protein (GFP) as a reporter. Promoter activity assay in zebrafish shows that the 0.2-kb fragment containing GC-box, CAAT-box, and TATA-box can direct tissue-specific GFP expression, while the 0.4-kb and 1.2-kb fragments with further upstream sequence included drive GFP expression more efficiently. We produced 1fngEGFP-transgenic founders showing somite-specific expression of GFP and consequently generated a hemizygous 1fngEGFP-transgenic line. The eggs from 1fngEGFP-transgenic female zebrafish show strong GFP expression, which is consistent to the reverse-transcription polymerase chain reaction PCR (RT-PCR) detection of 1fng transcripts in the fertilized eggs. This reveals that zebrafish 1fng is a maternal factor existing in matured eggs, suggesting that fish somitogcnesis may be influenced by maternal factors. |
| 英文摘要 | Lunatic fringe (Lfng), one modulator of Notch signaling, plays an essential part in demarcation of tissues boundaries during animal early development, especially somitogenesis. To characterize the promoter of zebrafish 1fng and generate somite-specific transgenic zebrafish, we isolated the upstream regulatory region of zebrafish 1fng by blast search at the Ensembl genome database (http://www. ensembl.org) and analyzed the promoter activity using green fluorescent protein (GFP) as a reporter. Promoter activity assay in zebrafish shows that the 0.2-kb fragment containing GC-box, CAAT-box, and TATA-box can direct tissue-specific GFP expression, while the 0.4-kb and 1.2-kb fragments with further upstream sequence included drive GFP expression more efficiently. We produced 1fngEGFP-transgenic founders showing somite-specific expression of GFP and consequently generated a hemizygous 1fngEGFP-transgenic line. The eggs from 1fngEGFP-transgenic female zebrafish show strong GFP expression, which is consistent to the reverse-transcription polymerase chain reaction PCR (RT-PCR) detection of 1fng transcripts in the fertilized eggs. This reveals that zebrafish 1fng is a maternal factor existing in matured eggs, suggesting that fish somitogcnesis may be influenced by maternal factors. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biotechnology & Applied Microbiology; Marine & Freshwater Biology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 关键词[WOS] | GFP REPORTER GENE ; SOMITE SEGMENTATION ; TRANSGENIC ZEBRAFISH ; SIGNALING MOLECULE ; DYNAMIC EXPRESSION ; NERVOUS-SYSTEM ; RADICAL FRINGE ; NOTCH ; SOMITOGENESIS ; DORSAL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239656600004 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8878]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jing,Sun, Yong-Hua,Wang, Na,et al. Upstream regulatory region of zebrafish lunatic fringe: Isolation and promoter analysis[J]. MARINE BIOTECHNOLOGY,2006,8(4):357-365. |
| APA | Liu, Jing,Sun, Yong-Hua,Wang, Na,Wang, Ya-Ping,&Zhu, Zuo-Yan.(2006).Upstream regulatory region of zebrafish lunatic fringe: Isolation and promoter analysis.MARINE BIOTECHNOLOGY,8(4),357-365. |
| MLA | Liu, Jing,et al."Upstream regulatory region of zebrafish lunatic fringe: Isolation and promoter analysis".MARINE BIOTECHNOLOGY 8.4(2006):357-365. |
入库方式： OAI收割
来源：水生生物研究所
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