Genetic structure and low-genetic diversity suggesting the necessity for conservation of the Chinese Longsnout catfish, Leiocassis longirostris (Pisces : Bagriidae)
文献类型：期刊论文
| 作者 | Wang, ZW; Zhou, JF; Ye, YZ; Wei, QW; Wu, QJ |
| 刊名 | ENVIRONMENTAL BIOLOGY OF FISHES
 |
| 出版日期 | 2006-04-01 |
| 卷号 | 75期号:4页码:455-463 |
| 关键词 | Leiocassis longirostris mitochondrial DNA genetic diversity PCR-RFLP |
| ISSN号 | 0378-1909 |
| 通讯作者 | Wang, ZW, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Genetic variation and phylogenetic relationship of Leiocassis longirostris populations from the Yangtze River were investigated at mitochondrial DNA level. The samples were collected from the upstream and mid-downstream of the Yangtze River. Three mitochondrial DNA fragments, ND5/6, cytochrome b (Cyt b) and control region (D-loop), were amplified and then digested by 10 restriction endonucleases. Twenty-three D-loop fragments randomly selected were sequenced. Digestion patterns of ND5/6 by AluI and HaeIII, D-loop by HinfI and RsaI, and Cyt b by HaeIII were polymorphic. Ten and eighteen haplotypes were obtained from RFLP data and sequence data, respectively. The individuals from upstream and mid-downstream of the Yangtze River were apparently divided into two groups. The average genetic distance was 0.008 and 0.010 according to the two data. Low diversities and decreasing abundance indicated that Leiocassis longirostris may be in severe danger and reasonable measures of fishery management should be taken. |
| 英文摘要 | Genetic variation and phylogenetic relationship of Leiocassis longirostris populations from the Yangtze River were investigated at mitochondrial DNA level. The samples were collected from the upstream and mid-downstream of the Yangtze River. Three mitochondrial DNA fragments, ND5/6, cytochrome b (Cyt b) and control region (D-loop), were amplified and then digested by 10 restriction endonucleases. Twenty-three D-loop fragments randomly selected were sequenced. Digestion patterns of ND5/6 by AluI and HaeIII, D-loop by HinfI and RsaI, and Cyt b by HaeIII were polymorphic. Ten and eighteen haplotypes were obtained from RFLP data and sequence data, respectively. The individuals from upstream and mid-downstream of the Yangtze River were apparently divided into two groups. The average genetic distance was 0.008 and 0.010 according to the two data. Low diversities and decreasing abundance indicated that Leiocassis longirostris may be in severe danger and reasonable measures of fishery management should be taken. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Ecology; Marine & Freshwater Biology |
| 类目[WOS] | Ecology ; Marine & Freshwater Biology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 关键词[WOS] | MITOCHONDRIAL-DNA VARIATION ; POLYMERASE-CHAIN-REACTION ; SEQUENCE VARIATION ; CONTROL REGION ; YANGTZE-RIVER ; FRESH-WATER ; POPULATIONS ; DIFFERENTIATION ; PHYLOGENY ; PATTERNS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000238741000010 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8928]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Jinzhou 434000, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, ZW,Zhou, JF,Ye, YZ,et al. Genetic structure and low-genetic diversity suggesting the necessity for conservation of the Chinese Longsnout catfish, Leiocassis longirostris (Pisces : Bagriidae)[J]. ENVIRONMENTAL BIOLOGY OF FISHES,2006,75(4):455-463. |
| APA | Wang, ZW,Zhou, JF,Ye, YZ,Wei, QW,&Wu, QJ.(2006).Genetic structure and low-genetic diversity suggesting the necessity for conservation of the Chinese Longsnout catfish, Leiocassis longirostris (Pisces : Bagriidae).ENVIRONMENTAL BIOLOGY OF FISHES,75(4),455-463. |
| MLA | Wang, ZW,et al."Genetic structure and low-genetic diversity suggesting the necessity for conservation of the Chinese Longsnout catfish, Leiocassis longirostris (Pisces : Bagriidae)".ENVIRONMENTAL BIOLOGY OF FISHES 75.4(2006):455-463. |
入库方式： OAI收割
来源：水生生物研究所
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