EST-based identification of genes expressed in the hypothalamus of male orange-spotted grouper (Epinephelus coioides)
文献类型：期刊论文
| 作者 | Zhou, Li; Yao, Bo; Xia, Wei; Li, Chuang-Ju; Wang, Yang; Shi, Yao-Hua; Gui, Jian-Fang |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2006-06-15 |
| 卷号 | 256期号:1-4页码:129-139 |
| 关键词 | expressed sequence tags hypothalamus grouper Epinephelus coioides sex inversion |
| ISSN号 | 0044-8486 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China |
| 中文摘要 | The orange-spotted grouper, Epinephelus coioides, is an important marine aquaculture fish, but its large-scale aquaculture has been hindered by the rarity of natural males because it is a protogynous hermaphroditic fish. Hypothalamus-pituitary-gonad is an important endocrine axis in regulating reproduction and sex differentiation. To reveal the molecular mechanism of hypothalamic physiological functions, we performed he studies on identification of genes expressed in the hypothalamus of male orange-spotted grouper using EST and RT-PCR strategy. A total of 1006 ESTs were sequenced, and 402 (39.96%) clones were identified as known genes and 604 (60.04%) as unknown genes. The 402 clones of known gene products represent transcripts of 18 1 genes. Moreover, the expression patterns of 26 unknown genes were analyzed in various tissues, such as liver, kidney, spleen, fat, heart, muscle, pituitary, hypothalamus, telencephalon, cerebellum, midbrain, medulla oblongata, ovary and testes. Five different categories of expression patterns were observed from them. Several unknown ESTs, such as DN551996, DN551998, DN552082, and DN552070, were detected to be hypothalamus-specific, brains-specific, or hypothalamus and gonad-specific genes. Interestingly, DN551996, not only exhibiting expression differences between ovary and testis, but also showing sex-dependent differences in hypothalamus of grouper, might play significant role in grouper reproduction or sex inversion. Further functional studies on these genes will provide more information on molecule regulation mechanism of sex inversion in groupers. (c) 2006 Elsevier B.V All fights reserved. |
| 英文摘要 | The orange-spotted grouper, Epinephelus coioides, is an important marine aquaculture fish, but its large-scale aquaculture has been hindered by the rarity of natural males because it is a protogynous hermaphroditic fish. Hypothalamus-pituitary-gonad is an important endocrine axis in regulating reproduction and sex differentiation. To reveal the molecular mechanism of hypothalamic physiological functions, we performed he studies on identification of genes expressed in the hypothalamus of male orange-spotted grouper using EST and RT-PCR strategy. A total of 1006 ESTs were sequenced, and 402 (39.96%) clones were identified as known genes and 604 (60.04%) as unknown genes. The 402 clones of known gene products represent transcripts of 18 1 genes. Moreover, the expression patterns of 26 unknown genes were analyzed in various tissues, such as liver, kidney, spleen, fat, heart, muscle, pituitary, hypothalamus, telencephalon, cerebellum, midbrain, medulla oblongata, ovary and testes. Five different categories of expression patterns were observed from them. Several unknown ESTs, such as DN551996, DN551998, DN552082, and DN552070, were detected to be hypothalamus-specific, brains-specific, or hypothalamus and gonad-specific genes. Interestingly, DN551996, not only exhibiting expression differences between ovary and testis, but also showing sex-dependent differences in hypothalamus of grouper, might play significant role in grouper reproduction or sex inversion. Further functional studies on these genes will provide more information on molecule regulation mechanism of sex inversion in groupers. (c) 2006 Elsevier B.V All fights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | CATFISH ICTALURUS-PUNCTATUS ; SALMON SALMO-SALAR ; CHANNEL CATFISH ; SEQUENCE TAGS ; SEX-CHANGE ; PROTOGYNOUS GROUPER ; TRANSCRIPTOME ANALYSIS ; BRAIN ; LIVER ; SPLEEN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000238394400015 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8950]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Li,Yao, Bo,Xia, Wei,et al. EST-based identification of genes expressed in the hypothalamus of male orange-spotted grouper (Epinephelus coioides)[J]. AQUACULTURE,2006,256(1-4):129-139. |
| APA | Zhou, Li.,Yao, Bo.,Xia, Wei.,Li, Chuang-Ju.,Wang, Yang.,...&Gui, Jian-Fang.(2006).EST-based identification of genes expressed in the hypothalamus of male orange-spotted grouper (Epinephelus coioides).AQUACULTURE,256(1-4),129-139. |
| MLA | Zhou, Li,et al."EST-based identification of genes expressed in the hypothalamus of male orange-spotted grouper (Epinephelus coioides)".AQUACULTURE 256.1-4(2006):129-139. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。