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Effects of age and dietary protein level on digestive enzyme activity and gene expression of Pelteobagrus fulvidraco larvae
文献类型：期刊论文
| 作者 | Wang, Chunfang; Xie, Shouqi; Zhu, Xiaoming; Lei, Wu; Yang, Yunxia; Liu, Hankang |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2006-04-28 |
| 卷号 | 254期号:1-4页码:554-562 |
| ISSN号 | 0044-8486 |
| 关键词 | Pelteobagrus fulvidraco pepsin trypsin amylase activity mRNA |
| 通讯作者 | Xie, SQ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | The present research studied the effects of age and dietary protein level on pepsin, trypsin and amylase activity and their mRNA level in Petteobagrus fulvidraco larvae from 3 to 26 days after hatch (DAH). Three DAH larvae were fed three isoenergetic diets, containing 42.8% (CP 43), 47.3% (CP 47) and 52.8% (CP 53) crude protein. Live food (newly hatched Artemia, unenriched) was included as a control. The effects of age on enzyme activity and mRNA were as follows: pepsin and trypsin activity in all treatment groups showed a significant (P < 0.05) increase at the beginning and decrease later although the timing of decrease was not the same among treatment groups and between the digestive enzymes. Pepsin and trypsin mRNA level followed the pattern of their respective enzyme changes. Age significantly affected amylase activity (P < 0.05) while age had no effect on amylase mRNA during the experimental period. The four diets significantly (P < 0.05) affected activity and mRNA level of pepsin and trypsin. Diets did not affect amylase activity or mRNA level. These results suggest that the effects of age on pepsin and trypsin gene expressions are at the transcriptional level. Dietary protein level does affect pepsin and trypsin gene expression in the early life of P. fulvidraco. There were no transcriptional effects on amylase gene expression. (c) 2005 Elsevier B.V. All rights reserved. |
| 英文摘要 | The present research studied the effects of age and dietary protein level on pepsin, trypsin and amylase activity and their mRNA level in Petteobagrus fulvidraco larvae from 3 to 26 days after hatch (DAH). Three DAH larvae were fed three isoenergetic diets, containing 42.8% (CP 43), 47.3% (CP 47) and 52.8% (CP 53) crude protein. Live food (newly hatched Artemia, unenriched) was included as a control. The effects of age on enzyme activity and mRNA were as follows: pepsin and trypsin activity in all treatment groups showed a significant (P < 0.05) increase at the beginning and decrease later although the timing of decrease was not the same among treatment groups and between the digestive enzymes. Pepsin and trypsin mRNA level followed the pattern of their respective enzyme changes. Age significantly affected amylase activity (P < 0.05) while age had no effect on amylase mRNA during the experimental period. The four diets significantly (P < 0.05) affected activity and mRNA level of pepsin and trypsin. Diets did not affect amylase activity or mRNA level. These results suggest that the effects of age on pepsin and trypsin gene expressions are at the transcriptional level. Dietary protein level does affect pepsin and trypsin gene expression in the early life of P. fulvidraco. There were no transcriptional effects on amylase gene expression. (c) 2005 Elsevier B.V. All rights reserved. |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | PENAEUS-VANNAMEI CRUSTACEA ; MESSENGER-RNA EXPRESSION ; DIFFERENT FOOD RATIONS ; WINTER FLOUNDER ; PLEURONECTES-AMERICANUS ; SOLEA-SENEGALENSIS ; CRASSOSTREA-GIGAS ; CLUPEA-HARENGUS ; COMPOUND DIETS ; ONTOGENY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000237838800058 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8962]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Shanghai Univ, Aquaculture Div, E Inst, Shanghai, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chunfang,Xie, Shouqi,Zhu, Xiaoming,et al. Effects of age and dietary protein level on digestive enzyme activity and gene expression of Pelteobagrus fulvidraco larvae[J]. AQUACULTURE,2006,254(1-4):554-562. |
| APA | Wang, Chunfang,Xie, Shouqi,Zhu, Xiaoming,Lei, Wu,Yang, Yunxia,&Liu, Hankang.(2006).Effects of age and dietary protein level on digestive enzyme activity and gene expression of Pelteobagrus fulvidraco larvae.AQUACULTURE,254(1-4),554-562. |
| MLA | Wang, Chunfang,et al."Effects of age and dietary protein level on digestive enzyme activity and gene expression of Pelteobagrus fulvidraco larvae".AQUACULTURE 254.1-4(2006):554-562. |
入库方式： OAI收割
来源：水生生物研究所
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