Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress
文献类型：期刊论文
| 作者 | Zhou, BS; Liu, WH; Siu, WHL; O'Toole, D; Lam, PKS; Wu, RSS |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2006-05-01 |
| 卷号 | 77期号:2页码:136-142 |
| 关键词 | reactive oxygen species (ROS) spermatozoa duroquinone comet assay lipid peroxidation (LPO) common carp |
| ISSN号 | 0166-445X |
| 通讯作者 | Wu, RSS, City Univ Hong Kong, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China |
| 中文摘要 | Toxicity of many waterborne organic contaminants to aquatic organisms is mediated through oxidative damages resulting from the production of reactive oxygen species (ROS). Using duroquinone as a model ROS inducer, we carried out in vitro and in vivo experiments to test the hypothesis that reproduction in common carp (Cyprinus carpio) can be impaired through oxidative damage of their spermatozoa. In vitro exposure of fish spermatozoa to 0, 12.5, 25, 50, 100 and 200 mu M duroquinone for 2 h showed a significant increase in the level of ROS in a dose-dependant manner. Sperm motility was significantly reduced in all exposure groups, but lipid peroxidation (LPO) and DNA strand break (measured by comet assay) were only enhanced at 50 mu M and above. A significant decrease in subsequent hatching rate was recorded in all the exposure groups, despite fertilization rate was not affected. In the in vivo experiment, spermatozoa were collected 24 and 72 h after fish received intra-peritoneal injections of 1.0 and 10 mg kg(-1) body weight duroquinone. DNA damage was clearly evident in spermatozoa of all treatment groups after 72 h exposure, and ROS was significantly enhanced in the high concentration group. LPO however, remained unchanged in both treatment groups. The overall results of both our in vitro and in vivo experiments demonstrated that duroquinone can induce ROS production in spermatozoa, which may impair sperm quality and subsequently reproductive success through oxidative stress. (c) 2006 Elsevier B.V. All rights reserved. |
| 英文摘要 | Toxicity of many waterborne organic contaminants to aquatic organisms is mediated through oxidative damages resulting from the production of reactive oxygen species (ROS). Using duroquinone as a model ROS inducer, we carried out in vitro and in vivo experiments to test the hypothesis that reproduction in common carp (Cyprinus carpio) can be impaired through oxidative damage of their spermatozoa. In vitro exposure of fish spermatozoa to 0, 12.5, 25, 50, 100 and 200 mu M duroquinone for 2 h showed a significant increase in the level of ROS in a dose-dependant manner. Sperm motility was significantly reduced in all exposure groups, but lipid peroxidation (LPO) and DNA strand break (measured by comet assay) were only enhanced at 50 mu M and above. A significant decrease in subsequent hatching rate was recorded in all the exposure groups, despite fertilization rate was not affected. In the in vivo experiment, spermatozoa were collected 24 and 72 h after fish received intra-peritoneal injections of 1.0 and 10 mg kg(-1) body weight duroquinone. DNA damage was clearly evident in spermatozoa of all treatment groups after 72 h exposure, and ROS was significantly enhanced in the high concentration group. LPO however, remained unchanged in both treatment groups. The overall results of both our in vitro and in vivo experiments demonstrated that duroquinone can induce ROS production in spermatozoa, which may impair sperm quality and subsequently reproductive success through oxidative stress. (c) 2006 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Marine & Freshwater Biology; Toxicology |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | OXYGEN SPECIES PRODUCTION ; DNA-DAMAGE ; HUMAN SPERM ; COMET ASSAY ; IN-VITRO ; LIPID-PEROXIDATION ; INDIVIDUAL CELLS ; SEMINAL PLASMA ; MILL EFFLUENT ; WHITE SUCKER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000236917400003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8994]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.City Univ Hong Kong, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, BS,Liu, WH,Siu, WHL,et al. Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress[J]. AQUATIC TOXICOLOGY,2006,77(2):136-142. |
| APA | Zhou, BS,Liu, WH,Siu, WHL,O'Toole, D,Lam, PKS,&Wu, RSS.(2006).Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress.AQUATIC TOXICOLOGY,77(2),136-142. |
| MLA | Zhou, BS,et al."Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress".AQUATIC TOXICOLOGY 77.2(2006):136-142. |
入库方式： OAI收割
来源：水生生物研究所
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