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Two cathelicidin genes are present in both rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar)
文献类型：期刊论文
| 作者 | Chang, CI; Zhang, YA; Zou, J; Nie, P; Secombes, CJ |
| 刊名 | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
 |
| 出版日期 | 2006 |
| 卷号 | 50期号:1页码:185-195 |
| 关键词 | CYSTEINE PROTEINASE-INHIBITOR ANTIMICROBIAL PEPTIDES IDENTIFICATION LEUKOCYTES SEQUENCE FAMILY SERUM LIPOPOLYSACCHARIDE EXPRESSION RESISTANCE |
| ISSN号 | 0066-4804 |
| 通讯作者 | Secombes, CJ, Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Zool Bldg,Tillydrone Ave, Aberdeen AB24 2TZ, Scotland |
| 中文摘要 | Further to the previous finding of the rainbow trout rtCATH_1 gene, this paper describes three more cathelicidin genes found in salmonids: two in Atlantic salmon, named asCATH_1 and asCATH_2, and one in rainbow trout, named rtCATH_2. All the three new salmonid cathelicidin genes share the common characteristics of mammalian cathelicidin genes, such as consisting of four exons and possessing a highly conserved preproregion and four invariant cysteines clustered in the C-terminal region of the cathelin-like domain. The asCATH_1 gene is homologous to the rainbow trout rtCATH_1 gene, in that it possesses three repeat motifs of TGGGGGTGGC in exon IV and two cysteine residues in the predicted mature peptide, while the asCATH_2 gene and rtCATH_2 gene are homologues of each other, with 96% nucleotide identity. Salmonid cathelicidins possess the same elastase-sensitive residue, threonine, as hagfish cathelicidins and the rabbit CAP18 molecule. The cleavage site of the four salmonid cathelicidins is within a conserved amino acid motif of QKIRTRR, which is at the beginning of the sequence encoded by exon W. Two 36-residue peptides corresponding to the core part of rtCATH_1 and rtCATH_2 were chemically synthesized and shown to exhibit potent antimicrobial activity. rtCATH_2 was expressed constitutively in gill, head kidney, intestine, skin and spleen, while the expression of rtCATH_1 was inducible in gill, head kidney, and spleen after bacterial challenge. Four cathelicidin genes have now been characterized in salmonids and two were identified in hagfish, confirming that cathelicidin genes evolved early and are likely present in all vertebrates. |
| 英文摘要 | Further to the previous finding of the rainbow trout rtCATH_1 gene, this paper describes three more cathelicidin genes found in salmonids: two in Atlantic salmon, named asCATH_1 and asCATH_2, and one in rainbow trout, named rtCATH_2. All the three new salmonid cathelicidin genes share the common characteristics of mammalian cathelicidin genes, such as consisting of four exons and possessing a highly conserved preproregion and four invariant cysteines clustered in the C-terminal region of the cathelin-like domain. The asCATH_1 gene is homologous to the rainbow trout rtCATH_1 gene, in that it possesses three repeat motifs of TGGGGGTGGC in exon IV and two cysteine residues in the predicted mature peptide, while the asCATH_2 gene and rtCATH_2 gene are homologues of each other, with 96% nucleotide identity. Salmonid cathelicidins possess the same elastase-sensitive residue, threonine, as hagfish cathelicidins and the rabbit CAP18 molecule. The cleavage site of the four salmonid cathelicidins is within a conserved amino acid motif of QKIRTRR, which is at the beginning of the sequence encoded by exon W. Two 36-residue peptides corresponding to the core part of rtCATH_1 and rtCATH_2 were chemically synthesized and shown to exhibit potent antimicrobial activity. rtCATH_2 was expressed constitutively in gill, head kidney, intestine, skin and spleen, while the expression of rtCATH_1 was inducible in gill, head kidney, and spleen after bacterial challenge. Four cathelicidin genes have now been characterized in salmonids and two were identified in hagfish, confirming that cathelicidin genes evolved early and are likely present in all vertebrates. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Microbiology; Pharmacology & Pharmacy |
| 类目[WOS] | Microbiology ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Microbiology ; Pharmacology & Pharmacy |
| 关键词[WOS] | CYSTEINE PROTEINASE-INHIBITOR ; ANTIMICROBIAL PEPTIDES ; IDENTIFICATION ; LEUKOCYTES ; SEQUENCE ; FAMILY ; SERUM ; LIPOPOLYSACCHARIDE ; EXPRESSION ; RESISTANCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234988000024 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9046]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland 2.Res Inst, Chilung, Taiwan 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chang, CI,Zhang, YA,Zou, J,et al. Two cathelicidin genes are present in both rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar)[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,2006,50(1):185-195. |
| APA | Chang, CI,Zhang, YA,Zou, J,Nie, P,&Secombes, CJ.(2006).Two cathelicidin genes are present in both rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar).ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,50(1),185-195. |
| MLA | Chang, CI,et al."Two cathelicidin genes are present in both rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar)".ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 50.1(2006):185-195. |
入库方式： OAI收割
来源：水生生物研究所
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