中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR

文献类型:期刊论文

作者Yin, LY; Huang, JQ; Huang, WM; Li, DH; Liu, YD
刊名TOXICON
出版日期2005-12-15
卷号46期号:8页码:859-864
关键词microcystin-RR Arabidopsis thaliana suspension cells antioxidant system malondiadehyde glutathione superoxide dismutase catalase glutathione S-transferase
ISSN号0041-0101
通讯作者Liu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要Microcystins are cyclic heptapeptide hepatoxins produced by many species of cyanobacteria. The toxic effects and mechanism of microcystins on animals have been well studied both in vivo and in vitro. It was also reported that microcystins had adverse effects on plants. However, to our knowledge, there is no information about the toxic effects and mechanism of microcystins on plant suspension cells. In this study, Arabidopsis thaliana suspension cells were exposed to a range dose of microcystin-RR. Lipid peroxidation, a main manifestation of oxidative damage, was studied and a time- and dose-dependent increase in malondiadehyde was observed. In contrast, glutathione (GSH) levels in the cells decreased after 48 h treatment with 1 and 5 mg/L of microcystin-RR. The activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly after 48 h exposure to I and 5 mg/L of microcystin-RR, but glutathione S-transferase (GST) activity showed no difference compared with the control. These results clearly indicate that microcystin-RR is able to cause oxidative damage in A. thaliana suspension cells. Decrease of GSH content and increases of SOD and CAT activities reveal that the antioxidant system may play an important role in eliminating or alleviating the toxicity of microcystin-RR. The possible toxicity mechanism of microcystin-RR on the A. thaliana suspension cells is also discussed in this paper. (C) 2005 Elsevier Ltd. All rights reserved.
英文摘要Microcystins are cyclic heptapeptide hepatoxins produced by many species of cyanobacteria. The toxic effects and mechanism of microcystins on animals have been well studied both in vivo and in vitro. It was also reported that microcystins had adverse effects on plants. However, to our knowledge, there is no information about the toxic effects and mechanism of microcystins on plant suspension cells. In this study, Arabidopsis thaliana suspension cells were exposed to a range dose of microcystin-RR. Lipid peroxidation, a main manifestation of oxidative damage, was studied and a time- and dose-dependent increase in malondiadehyde was observed. In contrast, glutathione (GSH) levels in the cells decreased after 48 h treatment with 1 and 5 mg/L of microcystin-RR. The activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly after 48 h exposure to I and 5 mg/L of microcystin-RR, but glutathione S-transferase (GST) activity showed no difference compared with the control. These results clearly indicate that microcystin-RR is able to cause oxidative damage in A. thaliana suspension cells. Decrease of GSH content and increases of SOD and CAT activities reveal that the antioxidant system may play an important role in eliminating or alleviating the toxicity of microcystin-RR. The possible toxicity mechanism of microcystin-RR on the A. thaliana suspension cells is also discussed in this paper. (C) 2005 Elsevier Ltd. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题Pharmacology & Pharmacy; Toxicology
类目[WOS]Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]Pharmacology & Pharmacy ; Toxicology
关键词[WOS]GLUTATHIONE S-TRANSFERASES ; CYANOBACTERIAL TOXIN ; SUPEROXIDE-DISMUTASE ; PROTEIN PHOSPHATASES ; AQUATIC ORGANISMS ; SPRAY IRRIGATION ; RAT HEPATOCYTES ; HIGHER-PLANTS ; LR ; GROWTH
收录类别SCI
语种英语
WOS记录号WOS:000234316200004
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/9060]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Wuhan Bot Gargen, Wuhan 430074, Peoples R China
3.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430070, Peoples R China
推荐引用方式
GB/T 7714
Yin, LY,Huang, JQ,Huang, WM,et al. Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR[J]. TOXICON,2005,46(8):859-864.
APA Yin, LY,Huang, JQ,Huang, WM,Li, DH,&Liu, YD.(2005).Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR.TOXICON,46(8),859-864.
MLA Yin, LY,et al."Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR".TOXICON 46.8(2005):859-864.

入库方式: OAI收割

来源:水生生物研究所

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