Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR
文献类型：期刊论文
| 作者 | Yin, LY; Huang, JQ; Huang, WM; Li, DH; Liu, YD |
| 刊名 | TOXICON
 |
| 出版日期 | 2005-12-15 |
| 卷号 | 46期号:8页码:859-864 |
| 关键词 | microcystin-RR Arabidopsis thaliana suspension cells antioxidant system malondiadehyde glutathione superoxide dismutase catalase glutathione S-transferase |
| ISSN号 | 0041-0101 |
| 通讯作者 | Liu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Microcystins are cyclic heptapeptide hepatoxins produced by many species of cyanobacteria. The toxic effects and mechanism of microcystins on animals have been well studied both in vivo and in vitro. It was also reported that microcystins had adverse effects on plants. However, to our knowledge, there is no information about the toxic effects and mechanism of microcystins on plant suspension cells. In this study, Arabidopsis thaliana suspension cells were exposed to a range dose of microcystin-RR. Lipid peroxidation, a main manifestation of oxidative damage, was studied and a time- and dose-dependent increase in malondiadehyde was observed. In contrast, glutathione (GSH) levels in the cells decreased after 48 h treatment with 1 and 5 mg/L of microcystin-RR. The activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly after 48 h exposure to I and 5 mg/L of microcystin-RR, but glutathione S-transferase (GST) activity showed no difference compared with the control. These results clearly indicate that microcystin-RR is able to cause oxidative damage in A. thaliana suspension cells. Decrease of GSH content and increases of SOD and CAT activities reveal that the antioxidant system may play an important role in eliminating or alleviating the toxicity of microcystin-RR. The possible toxicity mechanism of microcystin-RR on the A. thaliana suspension cells is also discussed in this paper. (C) 2005 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Microcystins are cyclic heptapeptide hepatoxins produced by many species of cyanobacteria. The toxic effects and mechanism of microcystins on animals have been well studied both in vivo and in vitro. It was also reported that microcystins had adverse effects on plants. However, to our knowledge, there is no information about the toxic effects and mechanism of microcystins on plant suspension cells. In this study, Arabidopsis thaliana suspension cells were exposed to a range dose of microcystin-RR. Lipid peroxidation, a main manifestation of oxidative damage, was studied and a time- and dose-dependent increase in malondiadehyde was observed. In contrast, glutathione (GSH) levels in the cells decreased after 48 h treatment with 1 and 5 mg/L of microcystin-RR. The activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly after 48 h exposure to I and 5 mg/L of microcystin-RR, but glutathione S-transferase (GST) activity showed no difference compared with the control. These results clearly indicate that microcystin-RR is able to cause oxidative damage in A. thaliana suspension cells. Decrease of GSH content and increases of SOD and CAT activities reveal that the antioxidant system may play an important role in eliminating or alleviating the toxicity of microcystin-RR. The possible toxicity mechanism of microcystin-RR on the A. thaliana suspension cells is also discussed in this paper. (C) 2005 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Pharmacology & Pharmacy; Toxicology |
| 类目[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 研究领域[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 关键词[WOS] | GLUTATHIONE S-TRANSFERASES ; CYANOBACTERIAL TOXIN ; SUPEROXIDE-DISMUTASE ; PROTEIN PHOSPHATASES ; AQUATIC ORGANISMS ; SPRAY IRRIGATION ; RAT HEPATOCYTES ; HIGHER-PLANTS ; LR ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234316200004 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9060]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Gargen, Wuhan 430074, Peoples R China 3.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, LY,Huang, JQ,Huang, WM,et al. Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR[J]. TOXICON,2005,46(8):859-864. |
| APA | Yin, LY,Huang, JQ,Huang, WM,Li, DH,&Liu, YD.(2005).Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR.TOXICON,46(8),859-864. |
| MLA | Yin, LY,et al."Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR".TOXICON 46.8(2005):859-864. |
入库方式： OAI收割
来源：水生生物研究所
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