Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis
文献类型:期刊论文
作者 | Zhu, B; Liao, X; Shao, Z; Rosenthal, H; Chang, J |
刊名 | MOLECULAR ECOLOGY NOTES
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出版日期 | 2005-12-01 |
卷号 | 5期号:4页码:888-892 |
关键词 | Acipenser sinensis microsatellites Yangtze river |
ISSN号 | 1471-8278 |
通讯作者 | Chang, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7th So Rd E Lake, Wuhan 430072, Peoples R China |
中文摘要 | From (GATA)(n) and (AAAG)(n) enriched genomic libraries for the Chinese sturgeon (Acipenser sinensis), 50 primer pairs were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Forty-six primer pairs exhibited highly polymorphic with two to 11 alleles per locus, while the rest four displayed monomorphic. These markers yielded 246 alleles in a survey of eight specimens of wild A. sinensis. Average observed heterozygosity ranged from 0.13 to 1.00. These loci should provide sufficient levels of genetic diversity to allow parentage analysis for artificial stocking management and delineation of fine-scale population structure. |
英文摘要 | From (GATA)(n) and (AAAG)(n) enriched genomic libraries for the Chinese sturgeon (Acipenser sinensis), 50 primer pairs were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Forty-six primer pairs exhibited highly polymorphic with two to 11 alleles per locus, while the rest four displayed monomorphic. These markers yielded 246 alleles in a survey of eight specimens of wild A. sinensis. Average observed heterozygosity ranged from 0.13 to 1.00. These loci should provide sufficient levels of genetic diversity to allow parentage analysis for artificial stocking management and delineation of fine-scale population structure. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
学科主题 | Biochemistry & Molecular Biology; Ecology; Evolutionary Biology |
类目[WOS] | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
研究领域[WOS] | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000234051200056 |
公开日期 | 2010-10-13 |
源URL | [http://ir.ihb.ac.cn/handle/152342/9068] ![]() |
专题 | 水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文 |
作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.World Surg Conservat Soc, D-21629 New Wulmstorf, Germany |
推荐引用方式 GB/T 7714 | Zhu, B,Liao, X,Shao, Z,et al. Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis[J]. MOLECULAR ECOLOGY NOTES,2005,5(4):888-892. |
APA | Zhu, B,Liao, X,Shao, Z,Rosenthal, H,&Chang, J.(2005).Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis.MOLECULAR ECOLOGY NOTES,5(4),888-892. |
MLA | Zhu, B,et al."Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis".MOLECULAR ECOLOGY NOTES 5.4(2005):888-892. |
入库方式: OAI收割
来源:水生生物研究所
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