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Optimization of RP-HPLC analysis of low molecular weight organic acids in soil

文献类型:期刊论文

作者Ding, JH; Wang, XX; Zhang, TL; Li, QM; Luo, MB
刊名JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
出版日期2006
卷号29期号:1页码:99-112
ISSN号1082-6076
关键词low molecular weight organic acid optimization soil solution
通讯作者Wang, XX, Chinese Acad Sci, Inst Soil Sci, POB X 821,71 E Beijing Rd, Nanjing 20008, Peoples R China
中文摘要RP-HPLC analysis for low molecular weight organic acids in soil solution has been optimized. An Atlantis (TM) C-18 column was used for the analyses. An optimal determination for eleven organic acids in soil solution was found at room temperature (25 degrees C) and 220 nm detection wavelength, with a mobile phase of 10 mM KH2PO4 -CH3OH (955, pH 2.7), a flow rate of 0.8 mL/min and 10 mu L sample size. The detection limits ranged 3.2-619 ng/mL, the coefficients of variation ranged 1.3-4.6%, and the recoveries ranged 95.6-106.3% for soil solution with standard addition on the optimal conditions proposed.
英文摘要RP-HPLC analysis for low molecular weight organic acids in soil solution has been optimized. An Atlantis (TM) C-18 column was used for the analyses. An optimal determination for eleven organic acids in soil solution was found at room temperature (25 degrees C) and 220 nm detection wavelength, with a mobile phase of 10 mM KH2PO4 -CH3OH (955, pH 2.7), a flow rate of 0.8 mL/min and 10 mu L sample size. The detection limits ranged 3.2-619 ng/mL, the coefficients of variation ranged 1.3-4.6%, and the recoveries ranged 95.6-106.3% for soil solution with standard addition on the optimal conditions proposed.
学科主题Biochemical Research Methods; Chemistry, Analytical
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]VARIABLE CHARGE SOILS ; CHROMATOGRAPHY ; DISSOLUTION ; KAOLINITE ; COMPLEXES ; ANIONS ; PLANTS
收录类别SCI
语种英语
WOS记录号WOS:000234138700008
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/9072]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位1.Chinese Acad Sci, Inst Soil Sci, Nanjing 20008, Peoples R China
2.E China Inst Technol, Fuzhou, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
4.E China Inst Technol, Fuzhou, Peoples R China
推荐引用方式
GB/T 7714
Ding, JH,Wang, XX,Zhang, TL,et al. Optimization of RP-HPLC analysis of low molecular weight organic acids in soil[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2006,29(1):99-112.
APA Ding, JH,Wang, XX,Zhang, TL,Li, QM,&Luo, MB.(2006).Optimization of RP-HPLC analysis of low molecular weight organic acids in soil.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,29(1),99-112.
MLA Ding, JH,et al."Optimization of RP-HPLC analysis of low molecular weight organic acids in soil".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 29.1(2006):99-112.

入库方式: OAI收割

来源:水生生物研究所

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