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Optimization of RP-HPLC analysis of low molecular weight organic acids in soil
文献类型:期刊论文
作者 | Ding, JH; Wang, XX; Zhang, TL; Li, QM; Luo, MB |
刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES |
出版日期 | 2006 |
卷号 | 29期号:1页码:99-112 |
ISSN号 | 1082-6076 |
关键词 | low molecular weight organic acid optimization soil solution |
通讯作者 | Wang, XX, Chinese Acad Sci, Inst Soil Sci, POB X 821,71 E Beijing Rd, Nanjing 20008, Peoples R China |
中文摘要 | RP-HPLC analysis for low molecular weight organic acids in soil solution has been optimized. An Atlantis (TM) C-18 column was used for the analyses. An optimal determination for eleven organic acids in soil solution was found at room temperature (25 degrees C) and 220 nm detection wavelength, with a mobile phase of 10 mM KH2PO4 -CH3OH (955, pH 2.7), a flow rate of 0.8 mL/min and 10 mu L sample size. The detection limits ranged 3.2-619 ng/mL, the coefficients of variation ranged 1.3-4.6%, and the recoveries ranged 95.6-106.3% for soil solution with standard addition on the optimal conditions proposed. |
英文摘要 | RP-HPLC analysis for low molecular weight organic acids in soil solution has been optimized. An Atlantis (TM) C-18 column was used for the analyses. An optimal determination for eleven organic acids in soil solution was found at room temperature (25 degrees C) and 220 nm detection wavelength, with a mobile phase of 10 mM KH2PO4 -CH3OH (955, pH 2.7), a flow rate of 0.8 mL/min and 10 mu L sample size. The detection limits ranged 3.2-619 ng/mL, the coefficients of variation ranged 1.3-4.6%, and the recoveries ranged 95.6-106.3% for soil solution with standard addition on the optimal conditions proposed. |
学科主题 | Biochemical Research Methods; Chemistry, Analytical |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
关键词[WOS] | VARIABLE CHARGE SOILS ; CHROMATOGRAPHY ; DISSOLUTION ; KAOLINITE ; COMPLEXES ; ANIONS ; PLANTS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000234138700008 |
公开日期 | 2010-10-13 |
源URL | [http://ir.ihb.ac.cn/handle/152342/9072] |
专题 | 水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文 |
作者单位 | 1.Chinese Acad Sci, Inst Soil Sci, Nanjing 20008, Peoples R China 2.E China Inst Technol, Fuzhou, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China 4.E China Inst Technol, Fuzhou, Peoples R China |
推荐引用方式 GB/T 7714 | Ding, JH,Wang, XX,Zhang, TL,et al. Optimization of RP-HPLC analysis of low molecular weight organic acids in soil[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2006,29(1):99-112. |
APA | Ding, JH,Wang, XX,Zhang, TL,Li, QM,&Luo, MB.(2006).Optimization of RP-HPLC analysis of low molecular weight organic acids in soil.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,29(1),99-112. |
MLA | Ding, JH,et al."Optimization of RP-HPLC analysis of low molecular weight organic acids in soil".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 29.1(2006):99-112. |
入库方式: OAI收割
来源:水生生物研究所
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