Molecular cloning and expression pattern of 14 kDa apolipoprotein in orange-spotted grouper, Epinephelus coioides
文献类型：期刊论文
| 作者 | Zhou, L; Wang, Y; Yao, B; Li, CJ; Ji, GD; Gui, JF |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2005-12-01 |
| 卷号 | 142期号:4页码:432-437 |
| 关键词 | apolipoproteins grouper orange-spotted grouper apo-14 embryogenesis yolk syncytial layer (YSL) liver morphogenesis expression pattern whole-mount in situ hybridization |
| ISSN号 | 1096-4959 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, Inst Hydrobiol, Wuhun Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | A novel fish-specific apolipoprotein (apo-14 kDa) has been recently cloned from eel and pufferfish. However, its expression pattern has not been elucidated. in this study, EcApo-14 has been screened from hypothalamic cDNA library of male orange-spotted grouper, which shows 62.9%, 51%, 46.9%, 43.2%, and 31.9% identities to Apo-14 of European flounder, pufferfish, Japanese eel, gibel carp, and grass carp, respectively. RT-PCR analysis reveals that this gene is first transcribed in neurula embryos and maintains a relatively stable expression level during the following embryogenesis. EcApo-14 transcripts are at a very high level during embryonic and early larval development in the yolk syncytial layer (YSL), and decrease in YSL and form intense staining in liver at 3 days after hatching. In adult tissues, EcApo-14 is predominantly expressed in liver and brain. The data suggested that EcApo-14 might play an important role in liver and brain morphogenesis and growth. (c) 2005 Elsevier Inc. All rights reserved. |
| 英文摘要 | A novel fish-specific apolipoprotein (apo-14 kDa) has been recently cloned from eel and pufferfish. However, its expression pattern has not been elucidated. in this study, EcApo-14 has been screened from hypothalamic cDNA library of male orange-spotted grouper, which shows 62.9%, 51%, 46.9%, 43.2%, and 31.9% identities to Apo-14 of European flounder, pufferfish, Japanese eel, gibel carp, and grass carp, respectively. RT-PCR analysis reveals that this gene is first transcribed in neurula embryos and maintains a relatively stable expression level during the following embryogenesis. EcApo-14 transcripts are at a very high level during embryonic and early larval development in the yolk syncytial layer (YSL), and decrease in YSL and form intense staining in liver at 3 days after hatching. In adult tissues, EcApo-14 is predominantly expressed in liver and brain. The data suggested that EcApo-14 might play an important role in liver and brain morphogenesis and growth. (c) 2005 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Zoology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Zoology |
| 关键词[WOS] | ZEBRAFISH ; GENE ; LIPOPROTEIN ; METABOLISM ; PROTEIN ; LIVER ; FISH ; PLASMA ; BETA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000233799400011 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9080]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, Wuhun Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, L,Wang, Y,Yao, B,et al. Molecular cloning and expression pattern of 14 kDa apolipoprotein in orange-spotted grouper, Epinephelus coioides[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2005,142(4):432-437. |
| APA | Zhou, L,Wang, Y,Yao, B,Li, CJ,Ji, GD,&Gui, JF.(2005).Molecular cloning and expression pattern of 14 kDa apolipoprotein in orange-spotted grouper, Epinephelus coioides.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,142(4),432-437. |
| MLA | Zhou, L,et al."Molecular cloning and expression pattern of 14 kDa apolipoprotein in orange-spotted grouper, Epinephelus coioides".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 142.4(2005):432-437. |
入库方式： OAI收割
来源：水生生物研究所
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