Vertical variation in dissolved alkaline phosphatase activity in a shallow eutrophic lake determined in reverse micelles
文献类型：期刊论文
| 作者 | Song, CL; Cao, XY; Li, JQ; Li, QM; Zhou, YY |
| 刊名 | JOURNAL OF FRESHWATER ECOLOGY
 |
| 出版日期 | 2005-12-01 |
| 卷号 | 20期号:4页码:627-634 |
| 关键词 | PROFILES PHOSPHORUS ENZYMES WATERS OCEAN |
| ISSN号 | 0270-5060 |
| 通讯作者 | Zhou, YY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | We evaluated the feasibility of microencapsulating dissolved alkaline phosphatase of a water body into reverse micelle systems prepared by hexadecyltrimethylammonium bromide as a surfactant in cyclohexane and 1-butanol as co-surfactant. The dissolved alkaline phosphatase activity within the micelle was described, including its kinetic parameters and the effects of pH and temperature on catalytic activity in surface, overlying and interstitial water of Lake Donghu. We found the similarities on the behavior of dissolved alkaline phosphatase of surface and interstitial water in reverse micelles, which was distinctly different from its behavior in the overlying water. This difference likely reflected the different origins of the dissolved alkaline phosphatase in the vertical profile of the lake. This system provides a novel tool with which to study the diversity and ecological significance of extracellular enzymes in aquatic environments. |
| 英文摘要 | We evaluated the feasibility of microencapsulating dissolved alkaline phosphatase of a water body into reverse micelle systems prepared by hexadecyltrimethylammonium bromide as a surfactant in cyclohexane and 1-butanol as co-surfactant. The dissolved alkaline phosphatase activity within the micelle was described, including its kinetic parameters and the effects of pH and temperature on catalytic activity in surface, overlying and interstitial water of Lake Donghu. We found the similarities on the behavior of dissolved alkaline phosphatase of surface and interstitial water in reverse micelles, which was distinctly different from its behavior in the overlying water. This difference likely reflected the different origins of the dissolved alkaline phosphatase in the vertical profile of the lake. This system provides a novel tool with which to study the diversity and ecological significance of extracellular enzymes in aquatic environments. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Ecology; Limnology |
| 类目[WOS] | Ecology ; Limnology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 关键词[WOS] | PROFILES ; PHOSPHORUS ; ENZYMES ; WATERS ; OCEAN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000233141200002 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9102]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, CL,Cao, XY,Li, JQ,et al. Vertical variation in dissolved alkaline phosphatase activity in a shallow eutrophic lake determined in reverse micelles[J]. JOURNAL OF FRESHWATER ECOLOGY,2005,20(4):627-634. |
| APA | Song, CL,Cao, XY,Li, JQ,Li, QM,&Zhou, YY.(2005).Vertical variation in dissolved alkaline phosphatase activity in a shallow eutrophic lake determined in reverse micelles.JOURNAL OF FRESHWATER ECOLOGY,20(4),627-634. |
| MLA | Song, CL,et al."Vertical variation in dissolved alkaline phosphatase activity in a shallow eutrophic lake determined in reverse micelles".JOURNAL OF FRESHWATER ECOLOGY 20.4(2005):627-634. |
入库方式： OAI收割
来源：水生生物研究所
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