Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group
文献类型：期刊论文
| 作者 | Jin, S; Hu, GA; Qian, YH; Zhang, L; Zhang, J; Qiu, G; Zeng, QT; Gui, JF |
| 刊名 | GENETICA
 |
| 出版日期 | 2005-11-01 |
| 卷号 | 125期号:2-3页码:223-230 |
| 关键词 | Dfak56 Drosophila melanogaster intron loss phylogenetic evolution single-copy nuclear gene |
| ISSN号 | 0016-6707 |
| 通讯作者 | Gui, JF, Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China |
| 中文摘要 | Intron loss and its evolutionary significance have been noted in Drosophila. The current study provides another example of intron loss within a single-copy Dfak gene in Drosophila. By using polymerase chain reaction (PCR), we amplified about 1.3 kb fragment spanning intron 5-10, located in the position of Tyr kinase (TyK) domain of Dfak gene from Drosophila melanogaster species group, and observed size difference among the amplified DNA fragments from different species. Further sequencing analysis revealed that D. melanogaster and D. simulans deleted an about 60 bp of DNA fragment relative to other 7 Drosophila species, such as D. elegans, D. ficusphila, D. biarmipes, D. takahashii, D. jambulina, D. prostipennis and D. pseudoobscura, and the deleted fragment located precisely in the position of one intron. The data suggested that intron loss might have occurred in the Dfak gene evolutionary process of D. melanogaster and D. simulans of Drosophila melanogaster species group. In addition, the constructed phylogenetic tree based on the Dfak TyK domains clearly revealed the evolutionary relationships between subgroups of Drosophila melanogaster species group, and the intron loss identified from D. melanogaster and D. simulans provides a unique diagnostic tool for taxonomic classification of the melanogaster subgroup from other group of genus Drosophila. |
| 英文摘要 | Intron loss and its evolutionary significance have been noted in Drosophila. The current study provides another example of intron loss within a single-copy Dfak gene in Drosophila. By using polymerase chain reaction (PCR), we amplified about 1.3 kb fragment spanning intron 5-10, located in the position of Tyr kinase (TyK) domain of Dfak gene from Drosophila melanogaster species group, and observed size difference among the amplified DNA fragments from different species. Further sequencing analysis revealed that D. melanogaster and D. simulans deleted an about 60 bp of DNA fragment relative to other 7 Drosophila species, such as D. elegans, D. ficusphila, D. biarmipes, D. takahashii, D. jambulina, D. prostipennis and D. pseudoobscura, and the deleted fragment located precisely in the position of one intron. The data suggested that intron loss might have occurred in the Dfak gene evolutionary process of D. melanogaster and D. simulans of Drosophila melanogaster species group. In addition, the constructed phylogenetic tree based on the Dfak TyK domains clearly revealed the evolutionary relationships between subgroups of Drosophila melanogaster species group, and the intron loss identified from D. melanogaster and D. simulans provides a unique diagnostic tool for taxonomic classification of the melanogaster subgroup from other group of genus Drosophila. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Genetics & Heredity |
| 类目[WOS] | Genetics & Heredity |
| 研究领域[WOS] | Genetics & Heredity |
| 关键词[WOS] | FOCAL ADHESION KINASE ; MOLECULAR EVOLUTION ; CARASSIUS-AURATUS ; GAIN ; RNA ; POLYMORPHISM ; TRANSFERRIN ; SEQUENCES ; SELECTION ; INSERTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000232841800010 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9118]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China 2.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, S,Hu, GA,Qian, YH,et al. Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group[J]. GENETICA,2005,125(2-3):223-230. |
| APA | Jin, S.,Hu, GA.,Qian, YH.,Zhang, L.,Zhang, J.,...&Gui, JF.(2005).Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group.GENETICA,125(2-3),223-230. |
| MLA | Jin, S,et al."Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group".GENETICA 125.2-3(2005):223-230. |
入库方式： OAI收割
来源：水生生物研究所
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