Cloning and analysis of 16 Rab genes from macronuclear DNA of Euplotes octocarinatus
文献类型：期刊论文
| 作者 | Zhi, H; Wang, W; Li, LY; Chai, BF; Sun, YH; Liang, AH |
| 刊名 | DNA SEQUENCE
 |
| 出版日期 | 2005-08-01 |
| 卷号 | 16期号:4页码:260-265 |
| 关键词 | Euplotes octocarinatus Rab cloning polymerase chain reaction |
| ISSN号 | 1042-5179 |
| 通讯作者 | Liang, AH, Shanxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China |
| 中文摘要 | Rab proteins belong to the largest family of the Ras superfamily of small GTPase that play an important role in intracellular vesicular traffic. So far, almost 60 members of Rab family have been identified in mammalian cells. To further study the diversity and function of Rab protein in evolution, unicellular protozoa ciliates, Euplotes octocarinatus, were used in this study, Rab genes were screened by PCR method from macronuclear DNA of E. octocarinatus. Sixteen Rab genes were obtained. They share 87.6 - 99.5% identities. Highly conserved GTP-binding domains were found. There are some hot regions that diverse sharply in these genes as well. |
| 英文摘要 | Rab proteins belong to the largest family of the Ras superfamily of small GTPase that play an important role in intracellular vesicular traffic. So far, almost 60 members of Rab family have been identified in mammalian cells. To further study the diversity and function of Rab protein in evolution, unicellular protozoa ciliates, Euplotes octocarinatus, were used in this study, Rab genes were screened by PCR method from macronuclear DNA of E. octocarinatus. Sixteen Rab genes were obtained. They share 87.6 - 99.5% identities. Highly conserved GTP-binding domains were found. There are some hot regions that diverse sharply in these genes as well. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biotechnology & Applied Microbiology; Genetics & Heredity |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 关键词[WOS] | BINDING ; GTPASES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000232134200003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9142]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Shanxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhi, H,Wang, W,Li, LY,et al. Cloning and analysis of 16 Rab genes from macronuclear DNA of Euplotes octocarinatus[J]. DNA SEQUENCE,2005,16(4):260-265. |
| APA | Zhi, H,Wang, W,Li, LY,Chai, BF,Sun, YH,&Liang, AH.(2005).Cloning and analysis of 16 Rab genes from macronuclear DNA of Euplotes octocarinatus.DNA SEQUENCE,16(4),260-265. |
| MLA | Zhi, H,et al."Cloning and analysis of 16 Rab genes from macronuclear DNA of Euplotes octocarinatus".DNA SEQUENCE 16.4(2005):260-265. |
入库方式： OAI收割
来源：水生生物研究所
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