中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Expression pattern and developmental behaviour of cellular nucleic acid-binding protein (CNBP) during folliculogenesis and oogenesis in fish

文献类型:期刊论文

作者Liu, JX; Gui, HF
刊名GENE
出版日期2005-08-15
卷号356期号:*页码:181-192
ISSN号0378-1119
关键词folliculogenesis oogenesis cyst formation granulosa cell gibel carp cellular nucleic acid-binding protein immunofluorescence
通讯作者Gui, HF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要In vertebrates, folliculogeneis establishes an intricate system for somatic cell-oocyte interaction, and ultimately leads to the acquisition of their respective competences. Although the formation process and corresponding interactions are strikingly similar in diverse organisms, knowledge of genes and signaling pathways involved in follicle formation is very incomplete and the underlying molecular mechanisms remain enigmatic. CNBP has been identified for more than ten years, and the highest level of CNBP transcripts has been observed in adult zebrafish ovary, but little is known about its functional significance during folliculogeneis and oogenesis. In this study, we clone CNBP cDNA from gibel carp (Carassius auratus gibelio), and demonstrate its predominant expression in gibel carp ovary and testis not only by RTPCR but also by Western blot. Its full-length cDNA is 1402 bp, and has an ORF of 489 nt for encoding a peptide of 163 aa. And its complete amino acid sequence shared 68.5%-96.8% identity with CNBPs from other vertebrates. Based on the expression characterization, we further analyze its expression pattern and developmental behaviour during folliculogeneis and oogenesis. Following these studies, we reveal an unexpected discovery that the CagCNBP is associated with follicular cells and oocytes, and significant distribution changes have occurred in degenerating and regenerating follicles. More interestingly, the CagCNBP is more highly expressed in some clusters of interconnected cells within ovarian cysts, no matter whether the cell clusters are formed from the original primordial germ cells or from the newly formed cells from follicular cells that invaded into the atretic oocytes. It is the first time to reveal CNBP relevance to folliculogeneis and oogenesis. Moreover, a similar stage-specific and cell-specific expression pattern has also been observed in the gibel carp testis. Therefore, further studies on CNBP expression pattern and developmental behaviour will be of significance for understanding functional roles of CNBP during gametogenests. (c) 2005 Elsevier B.V. All rights reserved.
英文摘要In vertebrates, folliculogeneis establishes an intricate system for somatic cell-oocyte interaction, and ultimately leads to the acquisition of their respective competences. Although the formation process and corresponding interactions are strikingly similar in diverse organisms, knowledge of genes and signaling pathways involved in follicle formation is very incomplete and the underlying molecular mechanisms remain enigmatic. CNBP has been identified for more than ten years, and the highest level of CNBP transcripts has been observed in adult zebrafish ovary, but little is known about its functional significance during folliculogeneis and oogenesis. In this study, we clone CNBP cDNA from gibel carp (Carassius auratus gibelio), and demonstrate its predominant expression in gibel carp ovary and testis not only by RTPCR but also by Western blot. Its full-length cDNA is 1402 bp, and has an ORF of 489 nt for encoding a peptide of 163 aa. And its complete amino acid sequence shared 68.5%-96.8% identity with CNBPs from other vertebrates. Based on the expression characterization, we further analyze its expression pattern and developmental behaviour during folliculogeneis and oogenesis. Following these studies, we reveal an unexpected discovery that the CagCNBP is associated with follicular cells and oocytes, and significant distribution changes have occurred in degenerating and regenerating follicles. More interestingly, the CagCNBP is more highly expressed in some clusters of interconnected cells within ovarian cysts, no matter whether the cell clusters are formed from the original primordial germ cells or from the newly formed cells from follicular cells that invaded into the atretic oocytes. It is the first time to reveal CNBP relevance to folliculogeneis and oogenesis. Moreover, a similar stage-specific and cell-specific expression pattern has also been observed in the gibel carp testis. Therefore, further studies on CNBP expression pattern and developmental behaviour will be of significance for understanding functional roles of CNBP during gametogenests. (c) 2005 Elsevier B.V. All rights reserved.
学科主题Genetics & Heredity
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Genetics & Heredity
研究领域[WOS]Genetics & Heredity
关键词[WOS]AURATUS-GIBELIO BLOCH ; ZINC-FINGER PROTEIN ; CARASSIUS-AURATUS ; MOUSE ; OOCYTE ; CELLS ; GENE ; CARP ; IDENTIFICATION
收录类别SCI
语种英语
WOS记录号WOS:000231750700020
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/9160]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan 430072, Peoples R China
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GB/T 7714
Liu, JX,Gui, HF. Expression pattern and developmental behaviour of cellular nucleic acid-binding protein (CNBP) during folliculogenesis and oogenesis in fish[J]. GENE,2005,356(*):181-192.
APA Liu, JX,&Gui, HF.(2005).Expression pattern and developmental behaviour of cellular nucleic acid-binding protein (CNBP) during folliculogenesis and oogenesis in fish.GENE,356(*),181-192.
MLA Liu, JX,et al."Expression pattern and developmental behaviour of cellular nucleic acid-binding protein (CNBP) during folliculogenesis and oogenesis in fish".GENE 356.*(2005):181-192.

入库方式: OAI收割

来源:水生生物研究所

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