Identification, cloning, and characterization of microsatellite DNA in Euglena gracilis
文献类型：期刊论文
| 作者 | Zhang, WJ; Yang, J; Yu, YH; Shen, YF |
| 刊名 | JOURNAL OF EUKARYOTIC MICROBIOLOGY
 |
| 出版日期 | 2005-07-01 |
| 卷号 | 52期号:4页码:356-359 |
| 关键词 | molecular markers protozoa RAMS RAPD Southern blot |
| ISSN号 | 1066-5234 |
| 通讯作者 | Yu, YH, Chinese Acad Sci, Inst Hydrobiol, Lab Protozoan Taxon & Ecol, Wuhan 430072, Peoples R China |
| 中文摘要 | Microsatellite DNA has been developed into one of the most popular genetic markers. We have identified and cloned microsatellite loci in the genome of a free-living protozoan Euglena gracilis FACHB-848, using the random amplified microsatellites method (RAMS). The digoxigenin-labelled oligonucleotides(CT)(10) and (GT)(10) served as probes to detect complementary sequences in the randomly amplified polymorphic DNA (RAPD) fingerprints produced by means of Southern blotting. Subsequently, positive RAPD fragments were cloned. From a total of 31 RAPD primer profiles, eight microsatellite loci of E. gracilis were detected and characterized. Further, six sites (i.e. EGMS1, EGMS3, EGMS4, EGMS5, EGMS6, and EGMS7) showed polymorphisms. We found a GT or CT microsatellite every 10.5 kb in the genome of E. gracilis, and similar to animal genomes, the (GT)(n) motif was much more abundant than the (CT)(n) motif. These polymorphic microsatellite DNA will serve as advantageous molecular markers for studying the genetic diversity and molecular ecology of Euglena. |
| 英文摘要 | Microsatellite DNA has been developed into one of the most popular genetic markers. We have identified and cloned microsatellite loci in the genome of a free-living protozoan Euglena gracilis FACHB-848, using the random amplified microsatellites method (RAMS). The digoxigenin-labelled oligonucleotides(CT)(10) and (GT)(10) served as probes to detect complementary sequences in the randomly amplified polymorphic DNA (RAPD) fingerprints produced by means of Southern blotting. Subsequently, positive RAPD fragments were cloned. From a total of 31 RAPD primer profiles, eight microsatellite loci of E. gracilis were detected and characterized. Further, six sites (i.e. EGMS1, EGMS3, EGMS4, EGMS5, EGMS6, and EGMS7) showed polymorphisms. We found a GT or CT microsatellite every 10.5 kb in the genome of E. gracilis, and similar to animal genomes, the (GT)(n) motif was much more abundant than the (CT)(n) motif. These polymorphic microsatellite DNA will serve as advantageous molecular markers for studying the genetic diversity and molecular ecology of Euglena. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | LINKAGE MAP ; MARKERS ; REPEATS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000230942000009 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/9196]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Lab Protozoan Taxon & Ecol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, WJ,Yang, J,Yu, YH,et al. Identification, cloning, and characterization of microsatellite DNA in Euglena gracilis[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2005,52(4):356-359. |
| APA | Zhang, WJ,Yang, J,Yu, YH,&Shen, YF.(2005).Identification, cloning, and characterization of microsatellite DNA in Euglena gracilis.JOURNAL OF EUKARYOTIC MICROBIOLOGY,52(4),356-359. |
| MLA | Zhang, WJ,et al."Identification, cloning, and characterization of microsatellite DNA in Euglena gracilis".JOURNAL OF EUKARYOTIC MICROBIOLOGY 52.4(2005):356-359. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。